中华耳科学杂志Issue(1)：142-146,5.DOI:10.3969/j.issn.1672-2922.2014.01.033
依达拉奉对噪声性聋防治作用
Edaravone protects against noise-induced hearing loss
摘要
Abstract
Objective To study effects of edaravone in preventing and protecting noise-induced hearing loss. Methods Thirty guinea pigs were randomly divided into 3 groups to receive no treatment,trearment with normal saling or treatment with edaravone given intrapertoneally at half an hour before noise exposure and for a week at 8.0mg/Kg.White niose (120dB SPL peak) were deliv-ered as noise exposure 4 hours for 4 days. Immediately after exposure to white noise and days 3,7following exposure, auditory brain-stem response(ABR) thresholds were measured.Outer hair cell morphological changes and succinate dehydrogenase(SDH) activity, immunohistochemical and scanning electron microscopy examined on Day 8 post-exposure. Results Three groups guinea pigs ABR threshold were no significant difference before exposured(P>0.05). Immediately after the noise, the saline group and edara-vone group ABR thresholds were higher than the normal control group, edaravone group ABR threshold was lower than the saline group(P<0.05). Three days later, edaravone group ABR threshold was significantly reduced and closed to the normal control group until 7 days,while the saline group was significantly higher than the control group, the difference was statistically significant (P<0.05).In the normal control group, there was deep SDH staining in the outer hair cells cytoplasmic ,and the nuclei and stereocilia ar-ranged in neat rows.Microscopy showed basement basal turn little SDH staining,cell swelling and irregular cell arrangement in the physiological saline treated animals.Whereas in the edaravone treated animals,there was deep SDH staining with signicantly re-duced cell and more regular cellular arrangement compared to physiological saline treated group. Mmunohistochemistry and scan-ning electron microscopy showed that normal control group hair cell and stereocilia arranged in neat rows,edaravone group hair cell and stereocilia loss were significantly less than the saline group. Conclusions Combined with previous experimental studies, and gives a preliminary presumption that edaravone has a great effect on the elimination of free radical in the noise-induce hearing loss.关键词
依达拉奉/噪声性聋/耳蜗Key words
Edaravone/Noise-induced hearing loss/Cochlea分类
医药卫生引用本文复制引用
吴龙军,黄益灯,于宁,夏思文,孙建和,郭维维,李兴启,陈立伟,杨仕明..依达拉奉对噪声性聋防治作用[J].中华耳科学杂志,2014,(1):142-146,5.基金项目
“全军十二五”面上项目(CWSlIJ256)，南京军区重大专项课题基金资助项目资助(10Z006，08Z007)，及国家自然科学基金项目（81241034,81271081）支持 （CWSlIJ256）
