广东海洋大学学报Issue(4)：11-16,6.
马氏珠母贝Su（H）基因克隆与组织特异性表达
Cloning and Tissue Specific Expression Analysis of Su (H) Gene in Pinctada martensii
摘要
Abstract
Su (H) gene (Suppressor of Hairless) is conservative in evolution and plays an important role in cell differentiation, proliferation and apoptosis. Based on the unigene sequence annotated as Su(H) in the transcriptome database of Pinctada martensii, using rapid-amplification of cDNA ends (RACE) technology, the full length of Su(H) gene from Pinctada martensii [pm-Su(H)] was obtained. The full length of pm-Su(H) cDNA was 3 830 bp, with an open reading frame (ORF) of 1 920 bp, encoding 640 amino acid residues. The 3' untranslated region (UTR) was 1 568 bp and the 5' UTR was 342 bp. Multiple sequence alignment indicated that Su(H) was highly conservative among other species and pm-Su(H) are most closely associated with Crassostrea gigas. Meanwhile, quantitative real-time PCR analysis demonstrated that pm-Su(H) mRNA was constitutively expressed in adductor muscle, gill, pearl sac, mantle, hepatopancreas, gonad, foot and hemocyte, with the most abundant mRNA in the sexual gland.关键词
马氏珠母贝/Su(H)基因/基因克隆/基因序列/基因表达/组织Key words
Pinctada martensii/Suppressor of Hairless gene/gene clone/gene sequence/gene expression/tissue分类
生物科学引用本文复制引用
焦钰,阎芳,杜晓东,黄荣莲,王庆恒,邓岳文..马氏珠母贝Su（H）基因克隆与组织特异性表达[J].广东海洋大学学报,2013,(4):11-16,6.基金项目
国家自然科学基金（31272635，31201023）；广东省自然科学基金（S2012040008042）；广东省教育厅育苗工程（2012LYM_0074）；广东海洋大学博士启动项目 （）
