广东海洋大学学报Issue(6)：14-23,10.
马氏珠母贝热休克蛋白HSP60基因的克隆与表达分析
Cloning and Express Characters of HSP60 gene from Pinctada martensii
摘要
Abstract
To clarify the role of Pinctada martensii HSP60 in the immune and resilience, its full length cDNA sequence has been cloned. The full length of HSP60 is 2495 bp, including 150 bp of 5’UTR, 611bp of 3’UTR, and 1734 bp of open reading frame. The open reading frame encodes a protein of 577 amino acids with an estimated molecular mass of 61.79 kDa and theoretical isoelectric point of 5.49. The alignment result shows that the homology of the nuclear acid sequence of HSP60 from P.martensii with that of the Crassostrea_gigas, Biomphalaria glabrata and other species was up to 75%. Analysis of the HSP60 amino acid sequence of P.martensii shows that it has a typical characteristic sequence of mt-HSP60, C-terminal typical repeat motifs GGM and an ATP binding domain. Results of qRT-PCR analysis show that the P. martensii HSP60 was ubiquitously expressed in adductor muscle, mantle, hemolymph, hepatopancreas, gonads and gills, with the highest expression level in hepatopancreas and the second highest level in gill. The HSP60 expression level was extremely low in hemolymph and adductor muscle. Statistical analysis indicates that upon the LPS stimulation, the transcription level of HSP60 mRNA began to increase and reached the highest level after 12 h, and then gradually declined to the normal level (P<0. 05).关键词
马氏珠母贝/HSP60/基因克隆/表达分析Key words
Pinctada martensii/HSP60/gene cloning/expression分类
生物科学引用本文复制引用
王志新,梁海鹰,杜晓东,黄荣莲,邓岳文,王庆恒,焦钰..马氏珠母贝热休克蛋白HSP60基因的克隆与表达分析[J].广东海洋大学学报,2013,(6):14-23,10.基金项目
国家自然科学基金（31272635）；广东省科技计划项目 （）
