广东海洋大学学报Issue(1):21-29,9.
盐度对莫荷罗非鱼幼鱼呼吸和氨氮排泄的影响
Effects of Salinity on Oxygen Consumption Rate and Ammonia Excretion Rate of Juvenile of Hybrid Tilapia (Oreochromis mossambicus × O. urolepis hornorum)
摘要
Abstract
The effects of salinities on the metabolic rate changes of a hybrid tilapia (Oreochromis mossambicus × O. urolepis hornorum) were investigated when salinities gradually increased with 6 every day from the fresh water to 12, 18, 24, 30 respectively. Oxygen consumption rate (RO) was measured by an intermittent flow respirometry in a controlled laboratory environment and ammonia excretion rate (RN) was determined by spectrophotometric analysis at 340 nm. The results showed that RO and RN of the hybrid tilapia increased with the rise of salinities, and increased first and then decreased with the extension of the time and got to the maximum values in each treatment at 5d, and the decline amplitudes were related with salinities. RO in salinities of 12, 18 dropped to the levels which have no significant difference with that in the control group (P>0.05) at 10 d, while significantly lower than that in the control group at 20 d (P<0.05). RO in salinities of 24, 30 decreased to the control levels and maintained stable (P>0.05) at 15 d. RN of juveniles increased with the rising salinity at 5 d and 10 d. At 15 d, RN decreased and maintained stable at the control level (P>0.05) in salinities 24 and 30. Also, in salinities 12 and 18, RN decreased to the control level at 15 d and were significantly lower than that in the other groups at 20 d (P<0.05). In the different salinities, the O:N ratio values were from 18.810 to 24.216 and ammonia quotients were from 0.083 to 0.108. It is suggested that hybrid tilapia juveniles utilize protein and lipid as main energy source, and the isotonic of hybrid tilapia will appeared when the salinity is from 12 to 18, and it is likely to be the optimal salinity for hybrid tilapia culture.关键词
莫荷罗非鱼/盐度/耗氧率/排氨率Key words
Oreochromis mossambicus×O. urolepis hornorum/salinity/oxygen consumption rate/ammonia excretion rate分类
农业科技引用本文复制引用
李明昊,陈刚,黄建盛,张健东,周晖,汤保贵,王忠良,卢迈新,李瑞伟..盐度对莫荷罗非鱼幼鱼呼吸和氨氮排泄的影响[J].广东海洋大学学报,2014,(1):21-29,9.基金项目
“十二五”国家科技支撑计划项目(2012BAD26B03);现代农业产业技术体系专项资金资助(CARS-49);广东省科技计划项目“营仔镇水产养殖与加工产品标准化建设”(2010B080203044);广东省专业镇中小微企业服务平台建设项目“公馆镇罗非鱼技术创新服务平台建设”(2012B091400016);国家农业科技成果转化资金项目“罗非鱼产业关键技术中试与示范”(2010GB2E000335);广东省海洋渔业科技推广专项项目“罗非鱼产业发展关键技术研究”(200899B02);广东省科技厅重大项目--省部产学研合作专项“罗非鱼产业推进关键技术研究与示范” ()