神经药理学报Issue(6)：16-21,6.
链脲佐菌素诱导大鼠糖尿病合并脑缺血损伤模型的建立
A Model of Cerebral Ischemia Injury in Streptozotocin-induced Diabetic Rats
摘要
Abstract
Objective:To establish a reliable model of focal cerebral ischemia injury in diabetic rats,and to explore the criteria for successful modeling. Methods: Forty healthy male SD rats were randomly divided into three groups: a normal sham-operation group,a normal cerebral ischemia-reperfusion injury group and a group of diabetes combined with cerebral ischemia-reperfusion injury. Type I diabetes was induced by an intraperitoneal injection of streptozotocin(STZ,60 mg·kg-1). The body mass and the level of fasting blood glucose in each group were measured on Weeks 1 and 4 after STZ injection. A focal cerebral ischemia-reperfusion model was prepared by a thread occlusion method four weeks after STZ treatment. The scores of neurological function were assessed and the percentage of infarct volume were calculated after 30 min ischemia followed by 24 h of reperfusion. Results: The body mass (P<0.001)was reduced and the fasting blood glucose(P<0.001)was increased significantly in diabetic rats than those in normal rats four weeks after STZ injection. The percentage of infarct volume(P<0.001)was increased more significantly and the brain injury appeared more severe in diabetic rats than in normal rats after reperfusion. The success rate of this model was 73%. Conclusion: In this study,we developed a model of focal cerebral ischemia injury in diabetic rats that demonstrates a high success rate and good reliability and stability.关键词
脑缺血/糖尿病/链脲佐菌素/大鼠/动物模型Key words
cerebral ischemia/diabetes mellitus/streptozotocin/rats/animal model分类
医药卫生引用本文复制引用
闫淼,王世蕊,张帆,郭吴,鲁茜,谷淑玲,印晓星,李梅..链脲佐菌素诱导大鼠糖尿病合并脑缺血损伤模型的建立[J].神经药理学报,2013,(6):16-21,6.基金项目
江苏省麻醉学重点实验室开放课题（No.KJS1107），江苏省高校研究生科研创新计划（No.CXZZ12_1000），徐州医学院药学院研究生科技创新计划（No.2011YXKYCX005），徐州医学院院长专项人才基金（No.2011KJ09），江苏省高校优势学科建设工程资助项目 （）
