浙江医学Issue(22)：1961-1963,3.
大鼠全脑照射后海马区VEGF表达变化及超微结构改变
Changes of VEGF expression and ultrastructure in hippocampus of adult rats after whole-brain irradiation
摘要
Abstract
Objective To investigate the changes of VEGF expression and ultrastructure in hippocampus of adult rats after whole- brain irradiation. Methods The whole brain of female Sprague- Dawley rats was irradiated by 20Gy 6mV X- ray. The ex-pression of VEGF mRNA in hippocampus tissue before and 1d, 1, 2, 4, 8w after ionizing radiation was detected by RT- PCR. The ultra- structural changes of hippocampus were observed by electronic microscope. Results RT- PCR showed a transient de-crease in VEGF mRNA expression within 1d after exposure to a single dose of 20Gy 6mV X- ray ionizing radiation;however, there were no differences in VEGF mRNA expression at 1, 2, 4, 8w after ionizing radiation when compared to controls (P>0.05). Neu-ronal mitochondria edema,vacuolization, endothelial gap increase and lumen narrow were demonstrated by electron microscope at 4w after whole brain irradiation. Conclusion VEGF may be involved in early radiation- induced damage in the hippocampus, which indicates that VEGF might be a novel target for prevention of radiation- induced brain injury.关键词
血管内皮生长因子/海马/脑损伤/放射疗法/大鼠/电子显微镜Key words
VEGF/Hippocampus/Brain injury/Radiation/Rat/Electron microscopy引用本文复制引用
杨伟芳,丁维军,孙晓南,朱敏,杨海华..大鼠全脑照射后海马区VEGF表达变化及超微结构改变[J].浙江医学,2013,(22):1961-1963,3.基金项目
浙江省自然科学基金项目 （）
