浙江医学Issue(22)：1978-1981,4.
PDGF对哮喘大鼠气道平滑肌细胞中ERK通路的影响研究
PDGF induces proliferation of airway smooth muscle cells of asthmatic rats through activation of ERK signal pathway
摘要
Abstract
Objective To investigate the effect of platelet- derived growth factor (PDGF)on proliferation of airway smooth muscle cells (ASMC) in asthmatic rats and its relation to external signal regulated kinase (ERK) signal pathway. Methods Asth-ma model was established in Sprague- Dawley rats. ASMCs separated from asthmatic rats were divided into four groups:group A without any intervention served as control, group B was treated with ERK blocking agent U0126 0.1μmol/L(B1), 1μmol/L (B2), 5μmol/L (B3) or 10μmol/L (B4), group C was treated with recombinant rat PDGF- BB 1μg/L (C1), 10μg/L (C2), 25μg/L (C3) or 50μg/L (C4), group D was treated with 10μmol/L U0126 and 50μg/L PDGF. The mRNA expressions of ERK1 and TGF- β1 in ASMCs of asthmatic rats were detected by RT- PCR. The protein expressions of ERK1 and TGF- β1 were detected by immuno-histochemistry (group A, B4, C4 and D only). Results Compared to group A, the expressions of ERK1 mRNA in ASMCs of groups B1, B2, B3 and B4 were significantly decreased(P<0.01) in a concentration- dependent manner;while the expressions of ERK1 mRNA in groups C1, C2, C3 and C4 were significantly increased (P<0.01) in a concentration- dependent manner. There was no significant difference in expressions of ERK1 mRNA between group D and group A (P>0.05). Immunohistochemistry showed that the expression of P- ERK1 in ASMCs of group B4 was significantly lower and that of group C4 was higher than that in group A (both P<0.01);while there was no significant difference between group D and group A (P>0.05). Conclusion PDGF can induce the proliferation of airway smooth muscle cells of asthmatic rats, in which the activation of ERK signal pathway is in-volved.关键词
哮喘/气道平滑肌细胞/细胞外调节激酶/气道重塑/转化生长因子- β1Key words
Asthma/Airway smooth muscle cels/External signal regulated kinase/Airway remodeling/TGF- β1引用本文复制引用
卢虹蓓,张维溪,李昌崇..PDGF对哮喘大鼠气道平滑肌细胞中ERK通路的影响研究[J].浙江医学,2013,(22):1978-1981,4.基金项目
国家自然科学基金项目（30571981；30271383) （）
