浙江医学Issue(22)：1992-1994,3.
人信号传导及转录激活因子shRNA载体的构建与鉴定
Construction and validation of shRNA vectors targeting human STAT1
摘要
Abstract
Objective To construct pGPU6 shRNA expression vector targeting human STAT1 gene and to evaluate its si-lencing effect in TZM- bl cellline. Methods The targeting sequences were designed and synthesized, and cloned into the pG-PU6/GFP/Neo vector. After confirmation by DNA sequencing, the recombinant vectors were transfected into TZM- bl cells, and the expression levels of STAT1 were determined. Results Four shRNA clones were successful y constructed and confirmed by DNA sequencing. The transfected cells presented gene silencing effect and the protein expression of STAT1 was down- regulat-ed by 63%. Conclusion We have successful y constructed STAT1- shRNA expression vector and its effect is validated in trans-fected TZM- bl cells.关键词
RNA干扰/人信号传导及转录激活因子1/小发夹状RNAKey words
RNA interference/STAT1/ShRNA引用本文复制引用
徐伶,杨宗兴,陈峰,虞容,杨劲,孟忠华..人信号传导及转录激活因子shRNA载体的构建与鉴定[J].浙江医学,2013,(22):1992-1994,3.基金项目
浙江省医药卫生科学研究基金项目 （）
