浙江医学Issue(3)：175-177,160,4.
帕立骨化醇对糖尿病肾病大鼠肾保护机制的研究
Renoprotective effect of paricalcitol in rats with diabetic nephropathy
摘要
Abstract
Objective To investigate the renoprotective effect of paricalcitol in rats with diabetic nephropathy (DN) and its mechanism. Methods Diabetic nephropathy was induced by intraperitoneal injection of streptozotocin in rats. The DN rats were randomly divided into DN group (group D) and paricalcitol group (group P), rats in group P were intraperitonealy injected with 0.4μg/kg paricalcitol t.i.w. Healthy non- DN rats served as control group (group N). After 12 weeks, 24h urinary protein and serum biochemical indicators were examined. Real- time PCR were used to detect expression of renin mRNA and cyclooxygenase- 2 (COX- 2) in renal tissue. Immunohistochemistry was used to detect the protein expression of COX- 2. ELISA method was used to detect the urine levels of prostaglandin E2 (PGE2). Results Compared to group N, 24- h urinary protein, serum creatinine(SCr), fasting blood glucose(FBG) and parathyroid hormone(PTH) levels and urinary PGE2 levels were significantly increased (P<0.05) in groups D and P;the expression of renin mRNA, COX- 2 mRNA and protein in renal tissue of groups D and P were also signifi-cantly increased (P<0.05). The above indexes of group D were markedly higher than those of group P (P<0.05). The renin level was positively correlated with urinary PGE2 (r=0.798, P<0.01) and COX- 2 mRNA expression (r=0.722, P<0.01). Conclusion Paricalcitol can significantly reduce the proteinuria in rats with diabetic nephropathy, which may be associated with down- regu-lation of COX- 2, PGE2 and renin expression.关键词
糖尿病肾病/帕立骨化醇/肾素/前列腺素E2/环氧化酶- 2Key words
Diabetic nephropathy/Paricalcitol/Renin/Prostaglandin E2/Cyclooxygenase- 2引用本文复制引用
高燕红,罗群,蔡珂丹,邬民香..帕立骨化醇对糖尿病肾病大鼠肾保护机制的研究[J].浙江医学,2014,(3):175-177,160,4.基金项目
浙江省医药卫生科技立项资助项目（2012KYB175）；宁波市自然科学基金资助项目 （）
