浙江医学Issue(7)：545-549,5.
黄芪甲苷对糖尿病肾病大鼠足细胞的影响及其机制研究
Effect of astragaloside IV on diabetic nephropathy rat podocytes and its mechanism
摘要
Abstract
Objective To investigate the protect effects of astragaloside IV(AS- IV) on diabetic nephropathy rat podocytes and explore its mechanism. Methods Healthy male Sprague- Dawley (SD) rats of 180~200g were randomly divided into normal control group(NC), diabetic nephropathy group(DN) and diabetic nephropathy with AS- IV treatment group(DN+AS- IV). DN was induced by intraperitoneal injection of streptozotocin. AS- IV treatment was started 2 weeks before STZ injection and lasted 14 weeks. 24 hour urine were col ected and 24 hour urinary protein were measured at the end of the 6th,12th week after STZ injec-tion. At the end of 6th and 12th week after STZ injection, rats were sacrificed and the blood samples were col ected for measuring biochemical parameters. The kidneys were harvested for histopathology, immunohistochemistry, electron microscopy and West-ern blot examinations, and the renal pathology, morphological changes of podocytes, podocyte density changes and expression ofα3β1 integrin protein were analyzed. Results In STZ- induced DN rats severe hyperglycemia and proteinuria were devel-oped. Mesangial expansion, increased podocyte loss and decreased integrin α3 and β1 expression were detected in DN rats. Treatment with AS- IV10mg/ (kg·d) for 14 weeks ameliorated proteinuria and podocyte foot process effacement, attenuated the loss of podocytes in glomerules and up- regulated the expression of integrinα3 andβ1 in podocytes. Conclusion AS- IV may protect podocyte and ameliorate diabetic nephropathy by restoring the expression ofα3β1 integrin in diabetic rats.关键词
黄芪甲苷/糖尿病肾病/足细胞/α3β1整合素Key words
Astragaloside IV/Diabetic nephropathy/Podocyte/α3β1 Integrin引用本文复制引用
罗蕴龄,陈宜方,何东元,陈建国..黄芪甲苷对糖尿病肾病大鼠足细胞的影响及其机制研究[J].浙江医学,2014,(7):545-549,5.基金项目
卫生部科研基金资助项目 （）
