浙江医学Issue(8)：656-658,3.
蝎毒抗癌多肽对HL-60细胞凋亡及Bcl-2和p53基因表达的影响
Effect of anticancer polypeptide in scorpion venom on apoptosis and Bcl-2, p53 gene expression in human leukemia HL-60 cells
庄海峰 1郑智茵 1庄晓芬 1周郁鸿 1沈建平1
作者信息
- 1. 310006 杭州，浙江中医药大学附属第一医院血液科
- 折叠
摘要
Abstract
Objective To investigate the effect of antitumor polypeptide extracted from scorpion venom (APBMV) on apoptosis and Bcl- 2, p53 gene expression in human leukemia HL- 60 cells. Methods APBMV was separated and purified by column chromatography with SephadexG- 50, and analyzed by HPLC method. The cultured HL- 60 cells were treated with differ-ent concentrations of APBMV. The cellgrowth was assayed by CCK- 8 method;the morphological changes of HL- 60 cells was observe by microscope, cellapoptosis was measured by flow cytometry, the expression of Bcl- 2 and p53 genes was detected by Western blot. Results CCK- 8 demonstrated that APBMV restrained HL- 60 cells multiplication in a dose- effect manner with a IC50 of 15.64μg/ml. Flow cytometer showed that APBMV induced HL- 60 cells apoptosis in a dose- effect manner;with APBMV (16μg/ml) for 48h, the apoptosis rate of HL- 60 cells was (36.1±2.1)%, significantly higher than that of control group. Western blot showed that APBMV down- regulated the expression of Bcl- 2 protein and up- regulated expression of p53 protein. Conclu-sion APBMV can effectively inhibit the proliferation and induce apoptosis of HL- 60 cells, which are associated with up- regula-tion of p53 and down- regulation of Bcl- 2 genes.关键词
蝎毒/HL- 60/细胞凋亡/Bcl- 2/p53Key words
Scorpion Venom/HL- 60/Apoptosis/Bcl- 2/P53引用本文复制引用
庄海峰,郑智茵,庄晓芬,周郁鸿,沈建平..蝎毒抗癌多肽对HL-60细胞凋亡及Bcl-2和p53基因表达的影响[J].浙江医学,2014,(8):656-658,3.
