浙江医学Issue(8)：659-662,4.
瘦素对大鼠肝纤维化星状细胞的作用及相关ERK信号转导机制
Effect of leptin on hepatic stellate cells in liver fibrosis rats and its relation to ERK signal transduction pathway
摘要
Abstract
Objective To investigate the effect of leptin on hepatic stel ate cells(HSC) in liver fibrosis rats and its relation to ERK signal transduction pathway. Methods The purified HSCs were obtained by the modified in situ perfusion and Optiprep density gradient centrifugation. The survival rate of HSCs was evaluated by trypan blue exclusion test, the identification of HSCs was tested by α- SMA immunocytochemical staining, and the morphological changes were observed by light microscope. The cultured HSCs were divided into four groups: the control group, the angiotensin II group (Ang II, 10-7mol/L), the Leptin group (Leptin, 100ng/ml), and the Leptin+Ang II group [Leptin (100ng/ml)+AngⅡ(10-7mol/L)]. The HSCs proliferation and col agen syn-thesis were measured by 3H- TdR and 3H- Pro incorporation methods. The protein levels of p- ERK1/ERK1, p- ERK2/ERK2 and Ang II were detected by Western blot. Results The survival rate of rat HSCs was>90%and the purity of HSCs was>95%after passage 1. The findings of HSCs proliferation and col agen synthesis showed that compared with control group, the proliferation and col agen synthesis of HSCs were significantly increased in the Ang II, Leptin and Leptin+Ang II groups (P<0.05);compared with the Ang II, Leptin groups, the HSCs proliferation and col agen synthesis were significantly increased in leptin+Ang II group (P<0.05). Western blot analysis showed that compared with the control group, the protein levels of p- ERK1/ERK1, p- ERK2/ERK2 and Ang II were significantly increased in Ang II, Leptin and Leptin+Ang II groups(P<0.05);compared with Ang II, Leptin groups, the protein levels of p- ERK1/ERK1, p- ERK2/ERK2 and Ang II were significantly increased in Leptin+Ang II group (P<0.05). Conclusion Leptin may up- regulate the Ang II levels, activate the ERK signal transduction pathway, stimulate the HSCs prolifera-tion and col agen synthesis, and then result in the liver fibrosis.关键词
瘦素/肝纤维化/HSC/ERK/信号转导机制Key words
Leptin/Hepatic fibrosis/HSC/ERK/Signal transduction mechanism引用本文复制引用
周光耀,金玲湘,林巍,潘陈为,诸葛璐,方佩佩..瘦素对大鼠肝纤维化星状细胞的作用及相关ERK信号转导机制[J].浙江医学,2014,(8):659-662,4.基金项目
温州市科技局基金项目 （）
