肿瘤药学Issue(5)：340-343,352,5.DOI:10.3969/j.issn.2095-1264.2013.084
PTEN在乳腺癌对顺铂化疗敏感性中的作用及其机制研究
Effects and Mechanisms of PTEN on the Cisplatin Sensitivity of Human MCF-7 Breast Cancer Cells
摘要
Abstract
Objective To study the roles of PTEN in the cisplatin chemosensitivity of breast cancer and its possible mecha-nisms. Methods PTEN-silenced MCF-7 cell line (MCF-7/PTENi) was established by lipofectamine transfection. The sensitiv-ity of MCF-7/PTENi cells to cisplatin was tested by MTT treated with or without ABT-737, a novel small molecule inhibitor of Bcl-2 protein. The expression of Bcl-2 was detected by Western blotting. Results The IC50 of MCF-7/PTENi cells treated by cisplatin was 16.01±3.03μM, and it was significantly higher than that of MCF-7/NC cells which was 7.86±0.85μM, with statistical difference (P=0.003). The expression of Bcl-2 in MCF-7/PTENi cells was significantly higher than that of MCF-7/NC cells (P=0.015). No significant difference was found between the MCF-7/PTENi and MCF-7/NC cells treated with 2μM ABT-737 and cisplatin (P=0.087). Conclusion Loss of PTEN expression decreased the chemosensitivity of breast cancer cells to cisplatin, and its mechanism may be involved with the increase of Bcl-2 expression.关键词
PTEN/乳腺癌/顺铂/化学治疗Key words
Phosphatase and tensin homolog (PTEN)/Breast cancer/Cisplatin/Chemotherapy分类
医药卫生引用本文复制引用
吴新刚,向安萍,陈杰..PTEN在乳腺癌对顺铂化疗敏感性中的作用及其机制研究[J].肿瘤药学,2013,(5):340-343,352,5.基金项目
湖南省教育厅基金资助项目（11C1284）。 （）
