肿瘤药学Issue(5)：348-352,5.DOI:10.3969/j.issn.2095-1264.2013.086
TGF-β1诱导的肝癌细胞SMMC-7721上皮间质转化对细胞Wnt/β-catenin表达的影响
A Study on the Changes of Wnt/β-Catenin Signaling Pathway by TGF-β1 Induced Epithelial Mesenchymal Transition in Hepatocellular Carcinoma Cell Line SMMC-7721
王勇 1范飞 2刘斌3
作者信息
- 1. 徐州医学院研究生院，江苏 徐州，221042
- 2. 连云港市东方医院普外科，江苏 连云港，222042
- 3. 第二军医大学东方肝胆外科医院胆道二科，上海，200438
- 折叠
摘要
Abstract
Objective To investigate the influence of TGF-β1-induced epithelial mesenchymal transition (EMT) on the ex-pression of Wnt/β-catenin in signaling pathway in human hepatocellular carcinoma cell line SMMC-7721. Methods EMT was induced by 5μg·mL-1 of TGF-β1 in SMMC-7721, and the mRNA and protein expressions of Wnt1,β-catenin and Vimen-tin were detected by RT-PCR and Western blot. Results TGF-β1 can strongly induce the EMT in hepatocellular carcinoma SMMC-7721, and the mRNA and protein expressions of Wnt1,β-catenin and Vimentin all remarkably increased (P<0.05). Conclusion EMT induced by TGF-β1 could promote the expression of Wnt/β-catenin in human hepatocellular carcinoma cell line SMMC-7721.关键词
肝癌/上皮间质转化/转化生长因子β1/Wnt/β-catenin信号通路Key words
Hepatocellular carcinoma/Epithelial mesenchymal transition/Transforming growth factor-β1 (TGF-β1)/Wnt/β-catenin signaling pathway分类
医药卫生引用本文复制引用
王勇,范飞,刘斌..TGF-β1诱导的肝癌细胞SMMC-7721上皮间质转化对细胞Wnt/β-catenin表达的影响[J].肿瘤药学,2013,(5):348-352,5.
