肿瘤药学Issue(1)：32-35,4.DOI:10.3969/j.issn.2095-1264.2014.006
内源性 H2S 参与了肝癌细胞 HepG-2的耐热
Endogenous H2S Involved in Thermotolerance of HepG-2
摘要
Abstract
Objective To investigate whether endogenous hydrogen sulfide (H2S) involved in thermotolerance of HepG-2 (HepG-2/tt). Methods Flow cytometry with PI staining was used to measure the apoptosis of HepG-2 and HepG-2/tt after thermotolerance; Methylene blue Colorimetry was used to detect the production of hydrogen sulfide and the activity of en-dogenous synthases of HepG-2 and HepG-2/tt. Results After exposed in 43℃ for 24 hours, the apoptosis rate of HepG-2/tt cells was decreased significantly when compared with normal HepG-2 cells (P<0.01). In addition, the production of Hy-drogen sulfide and the activity of endogenous synthases of HepG-2/tt were significantly increased (P<0.01). Conclusions HepG-2/tt cells had greater heat tolerance than normal HepG-2 cells, and much more endogenous synthases activity and hydrogen sulfide.关键词
肝癌细胞/耐热肝癌细胞/硫化氢/凋亡Key words
HepG-2/HepG-2/thermotolerance/Hydrogen sulfide/Apoptosisand HepG-2/tt cells分类
医药卫生引用本文复制引用
王义刚,陶寅,周成芳,黄婷,贺红杰,唐小卿,秦春宏..内源性 H2S 参与了肝癌细胞 HepG-2的耐热[J].肿瘤药学,2014,(1):32-35,4.基金项目
国家自然科学基金项目（NO.81172365）。 （）
