肿瘤药学Issue(2)：112-116,5.DOI:10.3969/j.issn.2095-1264.2014.022
地榆正丁醇萃取层对人肝癌细胞株HepG2增殖和凋亡的影响及其机制
The Effects and Mechanisms of n-butanol Extract from Sanguisorba Officinalis L. on the Proliferation and Apoptosis of Human Liver Cancer HepG2 Cells
摘要
Abstract
Objective To study the effects and mechanisms of n-butanol solvent of extract tannin (15.36%) from the San-guisorba officinalis L. on the proliferation and apoptosis of human liver cancer HepG2 cells. Methods HepG2 cells were treated with different concentrations of n-butanol extract. The growth inhibition ratio of HepG2 cells was measured by MTT. The cell apoptotic rate and intracellular ROS were detected by flow cytometry (FCM). Results After being treated by different concentra-tions of n-butanol extract from Sanguisorba officinalis L. (100, 150, 200, 250, 300μg·mL-1) for 48 h, the proliferation of HepG2 cells was inhibited obviously, and the IC50 was 222.87μg·mL-1. Moreover, with the increase of n-butanol extract content from Sanguisorba officinalis L., the growth inhibition ratio increased gradually, which were all significantly higher than that of the blank control group (P<0.05). The cell apoptotic rate and intracellular ROS content were significantly higher in the HepG2 cells treated by different concentrations (200, 400, 600μg·mL-1) of n-butanol extract from Sanguisorba officinalis L. for 48h than those of the blank control group. In addition, with the increase of the concentrations of n-butanol extract from Sanguisorba of-ficinalis L., the cell apoptotic rate and intracellular ROS content increased gradually. Conclusion The n-butanol extract from Sanguisorba officinalis L. could suppress the proliferation and promote the apoptosis of HepG2 cells in a concentration-depend-ent manner, and this may be related to its promotion in intracellular ROS production.关键词
地榆/抗肿瘤/细胞抑制和凋亡/活性氧Key words
Sanguisorba officinalis L./Liver cancer/Proliferation/Apoptosis/Active oxygen (ROS)分类
医药卫生引用本文复制引用
宛春雷,柴军红,孙晓薇,郝婧玮,孙雪芳..地榆正丁醇萃取层对人肝癌细胞株HepG2增殖和凋亡的影响及其机制[J].肿瘤药学,2014,(2):112-116,5.基金项目
牡丹江师范学院制药工程专业人才培养基金资助项目（编号2013JCZX0063）。 （）
