中南民族大学学报（自然科学版）Issue(3)：18-21,4.
烟碱降解菌 DAB2菌株的筛选、鉴定和降解特性
Isolation and Characterization of a Nicotine-Degrading Bacterium
摘要
Abstract
A nicotine-degrading bacterium , strain DAB2, was isolated from the tobacco field soil , using nicotine as the sole source of carbon.Based on standard morphologica1, physiological properties and 16S rRNA gene sequence, it was identified as Ochrobactrum intermedium.With initial nicotine concentration of 1.0g/L for 24 h, the strain DAB2 grew well and degraded 88.33% of nicotine .With 3.0g/L nicotine, 52.46% of nicotine was degraded .Both the growth and degradation declined with the degration of less than 10.36% when the nicotine concentration was more than 6.0g/L.Uv scanning of strain DAB2 cultured in 1.0 g/L nicotine at different time showed that single absorption peak existed at 259 nm in 0 h, peak height decreased at 259 nm gradually .And new peaks appealed at 230 nm and 290 nm in 8 h, indicating intermediate was produced .关键词
烟碱/生物降解/16S rDNA/中间苍白杆菌Key words
nicotine/biodegradation/16S rDNA/O.intermedium分类
生物科学引用本文复制引用
李晓华,杨晓潼,翟贵发,郭利,王晓丽..烟碱降解菌 DAB2菌株的筛选、鉴定和降解特性[J].中南民族大学学报（自然科学版）,2013,(3):18-21,4.基金项目
国家自然科学基金资助项目（31070087，30570046）；湖北省自然科学基金重点资助项目（2011CDA079）；国家大学生创新创业训练资助项目（GCX12012）；湖北省烟草公司面上资助项目 （）
