中外医学研究Issue(36)：1-2,3,3.
体外共培养环境对犬脂肪干细胞、口腔上皮细胞的影响
The Influence of Vitro Co-culture Environment on Canine Adipose Tissue Derived Stem Cell and Oral Keratinocyte
张钦 1田玉景 1程力1
作者信息
- 1. 南京医科大学附属无锡市人民医院 江苏 无锡 214023
- 折叠
摘要
Abstract
Objective:To investigate the influence of vitro co-culture environment on the migration and proliferation patterns of canine adipose-tissue derived stem cell and oral keratinocytes.Method:Obtain canine ADSC,OK and make the identification.Seed the two cell lines onto a scaled dish,record the migration rate of the two cell lines in co-culture environment,and compare it with the record in monoculture environment.Collect the supernatant of the two cell lines,and add it to the other’s culture medium to simulate a co-culture environment.Detect the changes in cell proliferation curves through MTT assay.Result:The migration rate of the ADSC and OK improved in co-culture environment.The proliferation curves of the two cell line were steeper in co-culture environment than in monoculture environment.Conclusion:In vitro co-culture environment,ADSC,OK exhibit mutual promotion,mutual attraction,collaborative proliferation situation,cell migration rate and proliferation rate are improved.They can be used together for the construction of the tissue engineering oral mucosa.关键词
脂肪干细胞/口腔上皮细胞/组织工程/共培养Key words
Adipose tissue derired stem/Oral keratinocyte/The tissue engineering/Co-culture environment分类
医药卫生引用本文复制引用
张钦,田玉景,程力..体外共培养环境对犬脂肪干细胞、口腔上皮细胞的影响[J].中外医学研究,2013,(36):1-2,3,3.
