组织工程与重建外科杂志Issue(4)：181-185,5.DOI:10.3969/j.issn.1673-0364.2013.04.001
胎猪BMSC体外构建软骨的实验研究
Porcine Fetal Bone Marrow Mesenchymal Stem Cells in Cartilage Tissue Engineering
摘要
Abstract
Objective To investigate the optimal seed cell for cartilage engineering by comparing the chondrogenesis capability of porcine fetal bone marrow mesenchymal stem cells (fBMSCs) and adult porcine BMSCs (aBMSCs). Methods Fetal pigs with gestational age of 70 days were obtained by the uterine-incision delivery, and primary fBMSCs were isolated from the bone marrow. Primary aBMSCs were isolated from the bone marrow which was aspirated from iliac. Cell morphology of the two kinds of cells at passage 3 were observed after in vitro proliferation. The two kinds of cells at passages 3 were characterized by their osteogenic, adipogenic and chondrogenic differentiation potential. Then the fBMSCs and aBMSCs were separately seeded onto a polyglycolic acid/polylactic acid (PGA/PLA) scaffold with the concentration of 1 ×108 cells/mL. All specimens were harvested after 8 weeks' culture in vitro. Gross observation, glycosaminoglycan (GAG) quantification, total collagen quantification and histology were used to compare related characteristic differences of engineered cartilage formed by the two kinds of cells. Results fBMSCs had better proliferation and multiple differentiation capacity than aBMSCs. The two kinds of cells both formed mature cartilage after 8 weeks of culture in vitro, and the engineered cartilage of aBMSCs group had better appearance. The GAG content and total collagen content of the cartilage formed by fBMSCs were both higher than the cartilage formed by aBMSCs (P<0.01). Histology and immunohistochemistry demonstrated that the cartilage formed by fBMSCs have more compact tissue structure. The cartilage matrix staining of cartilage formed by fBMSCs were stronger than that of cartilage formed by aBMSCs. Conclusion The fBMSCs seems to be the optimal seed cells for cartilage tissue engineering.关键词
骨髓间充质干细胞/胎猪/组织工程/软骨/种子细胞Key words
Bone marrow mesenchymal stem cells/Porcine fetal/Tissue engineering/Cartilage/Seed cell分类
生物科学引用本文复制引用
刘李娜,何爱娟,周广东,曹卫刚..胎猪BMSC体外构建软骨的实验研究[J].组织工程与重建外科杂志,2013,(4):181-185,5.基金项目
国家自然科学基金（81000677；31271046；50830105）。 （）
