组织工程与重建外科杂志Issue(5)：260-262,3.DOI:10.3969/j.issn.1673-0364.2013.05.005
大鼠Sertoli细胞对骨髓间充质干细胞体外诱导分化的影响
The Effects of Sertoli Cells on the Induced Differentiation of Rat Bone Marrow Mesenchymal Stem Cells
摘要
Abstract
Objective To explore the effects of Sertoli cells on the induced adipogenic, osteogenic and neuroblastic differentiation of bone marrow mesenchymal stem cells (BMSCs). Methods BMSCs were isolated and purified with the density gradient centrifugation method, Sertoli cells were isolated with two-step enzyme digestion;The adipogenic, osteogenic and neuroblastic differentiation of P3/P4 BMSCs were induced. Sertoli cells were co-cultured with BMSCs and then the morphological change of BMSCs were observed. Results After adipogenic induction, the lipid droplets that have strong refraction in the cytoplasm were observed by oil red O staining. The intracellular lipid droplets shrunk and the orange stained lipid droplets were hard to observe after adding Sertoli cells; After osteogenic induction, calcium nodules were shown by alizarin red S staining. More clearer calcium nodules were observed and the red-staining was stronger after adding Sertoli cells. No morphological change was observed on BMSCs before and after adding Sertoli cells. Conclusion Sertoli cells could promote BMSCs’ osteogenic differentiation, inhibit their adipogenic differentiation and have no effect on their neuroblastic differentiation.关键词
骨髓间充质干细胞/Sertoli细胞/诱导分化Key words
Bone marrow mesenchymal stem cells/Sertoli cells/Induced differentiation分类
生物科学引用本文复制引用
应涵汝,蒋黎华,王韦仑,李伟毅..大鼠Sertoli细胞对骨髓间充质干细胞体外诱导分化的影响[J].组织工程与重建外科杂志,2013,(5):260-262,3.基金项目
上海市卫生局科研计划（2009208）；上海市重点学科建设项目上海市免疫学研究所基金。 （）
