江苏大学学报（医学版）2014，Vol.24Issue(3)：221-225,5.DOI:10.13312/j.issn.1671-7783.y140033
MSCs旁分泌对多柔比星诱导的H9C2细胞凋亡的影响
Effect of hepatocyte growth factor on doxorubicin-induced H9C2 cells apoptosis
刘玲玲 1赵龙 1李晓丽 1张赢予 1王好 1周艳芳 1张国辉1
作者信息
- 1. 江苏大学附属人民医院心内科,江苏镇江212002
- 折叠
摘要
Abstract
Objective:To investigate the effect of hepatocyte growth factor (HGF) on doxorubicin (DOX)-induced H9C2 cells apoptosis.Methods:The third generation of mesenchymal stem cells(MSCs)were divided into three groups:cultured alone,HGF-siRNA-MSCs and NC-siRNA-MSCs.The concentration of HGF and transforming growth factor β1 (TGF-β1) were measured with both ELISA and Western-blot kit.H9C2 cells were exposed to 1.0 μmol/L doxorubicin for 4 hours.Then they were divided into four groups:cultured alone,co-cultured with MSCs,co-cultured with HGF-siRNA-MSCs and co-cultured with NC-siRNA-MSCs.After 24 hours,the apoptosis rate was determined by flow cytometry (FCM).Results:Compared with other groups,the concentration of TGF-β1 in MSCs were significantly increased in the HGF-siRNA-MSCs [(519.23 ± 24.34) pg/mL] than cultured alone [(459.65 ± 11.78) pg/mL] and NC-siRNA-MSCs [(459.33 ± 11.78) pg/mL,P ＜ 0.05].The apoptosis rate of H9C2 cells was increased significantly in cocultured with HGF-siRNA-MSCs(18.54 ± 0.64) ％ than co-cultured with MSCs(6.65 ± 0.49) ％ and NCsiRNA-MSCs [(9.70 ± 1.62) ％,P ＜0.05].Conclusion:HGF prevented DOX-induced H9C2 cells apoptosis by inhibition of TGF-β1.关键词
骨髓间充质干细胞/旁分泌/肝细胞生长因子/转化生长因子-β1/细胞凋亡/多柔比星Key words
mesenchymal stem cells/ paracrine/ hepatocyte growth factor/ transforming growth factor β1 / apoptosis / doxorubicin分类
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刘玲玲,赵龙,李晓丽,张赢予,王好,周艳芳,张国辉..MSCs旁分泌对多柔比星诱导的H9C2细胞凋亡的影响[J].江苏大学学报（医学版）,2014,24(3):221-225,5.
