中国中西医结合杂志2014，Vol.34Issue(6)：704-709,6.DOI:10.7661/CJIM.2014.06.0704
四君子汤对胃癌细胞株SGC-7901侧群细胞增殖的影响
Effect of Sijunzi Decoction on the Proliferation of Side Population Cells of Human Gastric Cancer Cell Line SGC-7901
摘要
Abstract
Objective To observe the proliferation changes of the side population of gastric cancer cell line SGC-7901 cells (SP),the non-side population (NSP) cells,and unsorted cells (Total) after intervened by Sijunzi Decoction (SD) containing serum.Methods Sixteen pure bred New Zealand rabbits were equally divided into the normal control group,the low dose SD group (at the daily dose of 7 mL/kg),the middle dose SD group (at the daily dose of 14 mL/kg),and the high dose SD group (at the daily dose of 28 mL/kg) according to the random digit table.Rabbits' serum was extracted after equal volume of corresponding medication was given by gastrogavage twice daily for 2 consecutive weeks.The drug serum was identified using high performance liquid chromatography.SP cells of SGC-7901 were detected using flow cytometry,SP and NSP cells were screened.The proliferation curve of SP,NSP,and Total cells were detected with CCK-8 assay.Changes of their proliferation were also observed.Results Ginsenoside Rg1,an effective ingredient in SD was detected in prepared drug serum.The proliferation of SGC-7901 SP cells was significantly higher than that of NSP cells and Total cells (P ＜0.05).Drug serum on gastric cancer cell line SGC-7901 SP,NSP,and Total cells could inhibit their proliferation,but its inhibition on SP cells' proliferation was significantly lower than on NSP and Total cells (P ＜ 0.05).Conclusions SD could significantly inhibit the proliferation of gastric cancer cell line SGC-7901 SP,NSP,and Total cells.But there exist obvious difference in the inhibition among the three groups.关键词
四君子汤/胃癌/侧群细胞/细胞增殖Key words
Sijunzi Decoction/ gastric cancer/ side population cells/ cell proliferation引用本文复制引用
李靖,钱军,贾建光,金鑫,喻大军,谢波,钱立宇,张立功,郭晨旭..四君子汤对胃癌细胞株SGC-7901侧群细胞增殖的影响[J].中国中西医结合杂志,2014,34(6):704-709,6.基金项目
国家自然科学基金资助项目(No.8117386) （No.8117386）
