中国中西医结合杂志2014，Vol.34Issue(6)：714-721,8.DOI:10.7661/CJIM.2014.06.0714
化瘀通络中药对糖尿病肾病大鼠肾皮质血管紧张素转化酶2-血管紧张素(1-7)-Mas轴的影响
Effect of Chinese Herbs for Stasis Removing and Collaterals Dredging upon Angiotensin-Converting Enzyme 2-Angiotensin-(1-7)-Mas Axis in the Renal Cortex of Diabetic Nephropathy Rats
摘要
Abstract
Objective To observe the effect of Chinese herbs for stasis removing and collaterals dredging (CHSRCD) upon angiotensin-converting enzyme 2-angiotensin-(1-7)-Mas axis in the renal cortex of diabetic nephropathy rats.Methods Totally 89 male Sprague-Dawley rats were randomly divided into the blank control group (C group,n =22),the high-glucose high-fat control group (H group,n =10),and the streptozotocin (STZ)-injecting group (n =57).The diabetes rat model (n =50) was induced by feeding high-glucose high-fat diet in combination with intraperitoneal injection of STZ,which were further divided into the model group (M group,n =24),the irbesartan group (I group,n =13),and the CHSRCD (Z group,n =13).Rats in I and Z groups were intragastrically fed with suspension of irbesartan and CHSRCD,once daily for 16 weeks.Equal volume of drinking water was administrated to rats in the rest groups.Blood glucose and 24 h urine protein quantitation were tested at four time points.And the mRNA expression of ACE2 and Mas at various time points was detected by Real-time PCR,immunohistochemical assay,and Western blot.Quantitative analyses of ACE2 and Mas protein expression were performed at the end of week 16.Results Compared with the C group,blood glucose increased in the H and M groups (P ＜0.01).It was higher in the H group (P ＜0.01).24 h urine protein quantitation at different time points increased in the M group,and it was higher than that in the H group (P ＜0.05).Compared with the M group,24 h urine protein quantitation decreased at the end of week 8 in the I group,and at the end of week 8 and 16 in the Z group (P ＜0.05).It was lower in the Z group than in the I group at the end of week 16 (P ＜0.05).Compared with the C and H groups,the expression of ACE2 mRNA in the renal cortex was lower in the M group at the end of week 16 (P ＜0.01).Compared with the M group,it was higher in the Z group (P ＜0.01).There was no statistical difference in the expressions of Mas mRNA at the end of week 16 between the C group and the M group (P ＞0.05).It was lower in the M group than in the H group (P ＜0.05).It was higher in the Z group than in the M group (P ＜0.05),and higher than in the I group (P ＜0.05).The expression of ACE2 and Mas protein in the M group decreased as time went by.The expression quantitation of ACE2 and Mas protein at the end of week 16 was lower in the M group than in the C group (P ＜0.05).Compared with the M group,ACE2 expression of the Z group and Mas of the I and Z groups increased more significantly (P ＜0.05).Conclusion CHSRCD could play a role in renal protection for diabetic nephropathy rats by up-regulating the mRNA and protein expression of ACE2 and Mas,promoting the ACE2-Ang-(1-7)-Mas axis,and lowering urinary protein.关键词
糖尿病肾病/化瘀通络/血管紧张素转化酶2/MasKey words
diabetic nephropathy/ stasis removing and collaterals dredging/angiotensin converting enzyme 2 / Mas引用本文复制引用
徐晶,马二卫,白璐,马赟,郭倩,贾蕊,张江华,陈志强..化瘀通络中药对糖尿病肾病大鼠肾皮质血管紧张素转化酶2-血管紧张素(1-7)-Mas轴的影响[J].中国中西医结合杂志,2014,34(6):714-721,8.基金项目
国家自然科学基金资助项目(No.81173419) （No.81173419）
