国际医药卫生导报2014，Vol.20Issue(13)：1838-1844,7.DOI:10.3760/cma.j.issn.1007-1245.2014.13.003
临床白念珠菌耐药性与ERG3基因突变及表达的关系
Correlation between the point mutations and high expression of ERG3 gene and drug resistance in clinical candida albicans
摘要
Abstract
Objective To investigate the correlation between the mutation and high expression of ERG3 to antifungal drug resistance in candida albicans.Methods Candida albicans was measured minimum inhibitory concentration (MIC) by microdilution susceptibility testing.DNA was extracted.PCR was carried out to amply the full-length ERG3.The purified ERG3 compared with known standard sequences in genbank (AF069572) through BLAST analysis.The mRNA expression of ERG3 was determined by reverse transcription-polymerase chain reaction (RT-PCR).Results Six mutations were detected in ERG3 gene of thirty-Six candida albicans strains.Nineteen strains appeared synonymous-mutations,including T51C,T432C,T381C,C438T,T1044C.Two strains appeared missense mutation,including C1052T.High expression rate of fluconazole resistant group was higher than that of the sensitive group.Conclusion The mutation and high expression of ERG3 genes increased antifungal drug resistance.Meanwhile,variety of regulating mechanism participate in the formation of drug resistance in candida albicans.关键词
白念珠菌/ERG3基因/耐药性Key words
Candida albicans/ ERG3gene/ Resistance引用本文复制引用
陈晋宇,冯文莉,杨静,奚志琴,乔祖莎..临床白念珠菌耐药性与ERG3基因突变及表达的关系[J].国际医药卫生导报,2014,20(13):1838-1844,7.基金项目
山西省基础研究项目资助(2012011045-3) （2012011045-3）
山西省青年科技研究基金(2012021035-3) （2012021035-3）
山西医科大学科技创新基金(01201013) （01201013）
山西省卫生厅科技攻关项目(201301009) （201301009）
