国际医药卫生导报2014，Vol.20Issue(16)：2467-2470,4.DOI:10.3760/cma.j.issn.1007-1245.2014.16.020
丙泊酚对内毒素诱导大鼠急性肺损伤及细胞凋亡的影响
Effect of propofol on lipopolysaccharide-induced acute lung injury and apoptosis in rats
林博 1高友光 1林财珠1
作者信息
- 1. 350004福州,福建医科大学附属第一医院麻醉科
- 折叠
摘要
Abstract
Object To investigate the effect of propofol on lipopolysaccharide (LPS)-induced acute lung injury and apoptosis in rats.Methods 18 SD male rats were randomly and equally divided into a control group,an LPS + normal saline (NS) group,and an LPS + propofol group.After anesthetization,the rats were intratracheally given a single dose of aerosolized LPS (1 mg/kg) and the control rats were intratracheally given normal saline.Then,the rats in the LPS + propofol group were iutravenously administrated propofol 5 mg/kg followed by continuous infusion 10 mg/(kg · h) for 6 hours.The rats in the control group and the LPS + NS group received a same volume of NS.All rats were sacrificed to detect interstitial edema and histopathology changes respectively by wet-to-dry weight ratio (W/D) and hematoxylin-eosin (HE) staining.To further investigate the protective effects of propofol on apoptosis,TUNEL staining,fluorometric assay,and western blot were respectively used for measuring the number of apoptotic cell,caspase-3 activity,and apoptosis relative proteins including Bax and Bcl-2.Results Comparing with the control group,the LPS + NS group got interstitial edema.exhibited siguificant lung injury characterizing by deterioration of histopathological characteristics,had a higher W/D,apoptosis in ALI significantly reflected by massive TUNEL staining-positive cells,higher caspase-3 activity (P ＜ 0.01),and up-regulation of Bax and down-regulation of Bcl-2 (P ＜ 0.01).After propofol treatment,these alterations were partially inhibited.Conclusions These results indicated that propofol treatment ameliorates LPS-induced ALI inhibiting apoptosis.关键词
内毒素/急性肺损伤/丙泊酚/凋亡Key words
Lipopolysaccharide/ Acute lung injury/ Propofol/ Apoptosis引用本文复制引用
林博,高友光,林财珠..丙泊酚对内毒素诱导大鼠急性肺损伤及细胞凋亡的影响[J].国际医药卫生导报,2014,20(16):2467-2470,4.
