江苏大学学报（医学版）2014，Vol.24Issue(4)：312-315,4.DOI:10.13312/j.issn.1671-7783.y140159
1株肠道病毒71型南京分离株的全基因组序列的测定及遗传进化分析
Sequence and phylogenetic analysis of an enterovirus 71 strain isolated in Nanjing
周晋 1曹喜华 2曹彤 1陈红兵1
作者信息
- 1. 南京医科大学附属南京儿童医院检验科,江苏南京210008
- 2. 南阳医学高等专科学校第一附属医院麻醉科,河南南阳473058
- 折叠
摘要
Abstract
Objective:To sequence and analyze the complete genome of an enterovirus 71 isolate (EV71-NJ2012) isolated in Nanjing(2012),and to compare the sequences deposited in GenBank.Methods:According to the genome information from GenBank,8 pairs of primer were designed to cover the whole genome.The genome sequences were determined by RT-PCR sequencing method,and the sequences were analyzed using bioinformatic software (MEGA 4.0 and DNAStar).Results:The full genomic length of EV71-NJ2012 was 7 404 bp,which contained 5'UTR (742 bp),the open reading frame (ORF,6 582 bp) and 3'UTR (82 bp).The ORF encoded a polyprotein with 2 193 amino acid residues.The viral sequences belong to the sub-type C4a genotype and had the maximum homology with Shanghai isolate,however,its sequence was quite different with the Taiwan and Hubei isolates.Conclusion:The genome structure of EV7 1-N J2012 strain is consistent with enterovirus.The phylogenetic relationships of the EV71-NJ2012 strain with other EV71 strains are different.EV71-NJ2012 strain has the maximum sequence homology with Shanghai isolate,and is quite different with the Taiwan and Hubei isolates.It maybe infer that EV71-NJ2012 and Shanghai strains probably originate from the same EV71 strain.关键词
手足口病/肠道病毒71型/序列分析Key words
hand-foot-mouth disease/ enterovirus 71 (EV71) / sequence analysis分类
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周晋,曹喜华,曹彤,陈红兵..1株肠道病毒71型南京分离株的全基因组序列的测定及遗传进化分析[J].江苏大学学报（医学版）,2014,24(4):312-315,4.
