江苏大学学报（医学版）2014，Vol.24Issue(4)：351-354,4.DOI:10.13312/j.issn.1671-7783.y140141
类风湿关节炎患者外周血单个核细胞中糖酵解相关基因的表达
Expression of glycolysis related gene in peripheral blood mononuclear cells of patients with rheumatoid arthritis
摘要
Abstract
Objective:To explore the mRNA expression and potential significance of glycolysis related gene in peripheral blood mononuclear cells(PBMC) of patients with rheumatoid arthritis(RA).Methods:PBMC were purified by Ficoll and glycolysis related genes mRNA expression was analysed by real-time PCR.Results:Compared with healthy control,PBMC from active RA patients significantly expressed higher level of glycolysis related genes including TPI,ENO,PKM and MCT.However,there were no significant differences in these genes between inactive group and control group.Conclusion:In RA patients,glycolysis related genes mRNA expression level were changed.Glycolysis may play an important role in the mechanism of RA.关键词
类风湿关节炎/定量PCR/糖酵解相关基因/外周血单个核细胞Key words
rheumatoid arthritis/ real-time PCR/ glycolysis related gene/ peripheral blood mononuclear cell分类
医药卫生引用本文复制引用
熊御云,王蓓,陶真,吴玲,尤海燕,王文红,焦志军..类风湿关节炎患者外周血单个核细胞中糖酵解相关基因的表达[J].江苏大学学报（医学版）,2014,24(4):351-354,4.基金项目
国家自然科学基金资助项目(81301657) （81301657）
镇江市卫生科技重点项目(SH2011051) （SH2011051）
