江苏大学学报（医学版）2014，Vol.24Issue(4)：359-362,368,5.DOI:10.13312/j.issn.1671-7783.y1400126
阿米洛利通过自噬-溶酶体途径保护PC12细胞
Amiloride protects PC12 cells from MPP + induced injury via autophagy-lysosome pathway
张润娉 1李建平1
作者信息
- 1. 苏州高新区人民医院神经内科,江苏苏州215129
- 折叠
摘要
Abstract
Objective:To evaluate the neuroprotective effects of amiloride; a non-selective blockers of acid-sensing ion channels,on PC12 cells and to view the influence of autophagy-lysosome pathway (ALP).Methods:PC12 cells were tested with methy-phenylpyridi(MPP+),while giving amiloride as interventiong:The cells viability was analyzed by MTT assay; The cells injury was assessed by lactate dehydrogenase (LDH) assay;Flow cytometry was used to study the apoptotic;Western blot analysis was used to study the autophagic mechanisms.Results:MPP + medium treatment resulted in significantly lower survival rate,the supernatant of LDH leakage was significantly higher,the apoptosis rate increased,the expression of LC3-Ⅱ was inhibited while induced more expression of LAMP2a ; but amiloride effectively improved the cell survival rate,reduced LDH leakage rate,inhibited apoptosis,and upregulated LC3-Ⅱ protein,which is associated decrease expression of LAMP2a.Conclusion:Amiloride can protect PC12 cells against MPP +-induced cell death by autophagic,through inhibition of acid-sensing ion channels activity.关键词
阿米洛利/PC12细胞/MPP+/自噬-溶酶体通路Key words
amiloride / PC12 cells / MPP + / autophagy-lysosome pathway分类
医药卫生引用本文复制引用
张润娉,李建平..阿米洛利通过自噬-溶酶体途径保护PC12细胞[J].江苏大学学报（医学版）,2014,24(4):359-362,368,5.
