中国药房2014，Vol.25Issue(35)：3293-3296,4.DOI:10.6039/j.issn.1001-0408.2014.35.10
HPLC法与UPLC法同时测定黄芩中5种黄酮类成分含量的比较
Comparison of HPLC and UPLC for the Determination of 5 Flavonoids in Scutellaria baicalensis
摘要
Abstract
OBJECTIVE:To provide simple and efficient method for the quality control of multiple componets in Scutellaria baicalensis.METHODS:HPLC and UPLC method were established to determine the contents of baicalin,wogonoside,baicalein,wogonin and oroxylin A in S.baicalensis.The separation,linear relationship,precision,reproducibility,stability and recovery were compared between 2 methods.For HPLC system,Diamonsil C18 (250 mm × 4.6 mm,5 μm) column was applied with mobile phase consisted of acetonitrile-water-formic acid (gradient elution) at the flow rate of 1.0 ml/min.The colunn temperature was 30 ℃,and detection wavelength was set at 280 nm.For UPLC system,Acquity BEH C18(50 mm×2.1 mm,1.7 μm) column was applied with mobile phase consisted of 0.1％ formic acid-acetonitrile (gradient elution) at the flow rate of 0.4 ml/min.The column temperature was set at 40 ℃,and detection wavelength was set 280 nm.RESULTS:Both methods had good linear relationship in sample size and peak area (r≥0.999 5) ; RSDs of precision,stability and reproducibility tests were lower than 4％ ; average recovery was higher than 96％ (RSD＜5％,n=6).CONCLUSIONS:Compared with HPLC method,UPLC method is faster and more sensitive and costs less solvents,and can be used for the quality control of various components in S.baicalensis simultaneously.关键词
黄芩/黄酮类成分/含量测定/高效液相色谱法/超高效液相色谱法Key words
Scutellaria baicalensis / Flavonoids/ Content determination/ HPLC/ UPLC分类
医药卫生引用本文复制引用
李化,刘静,董红敬,刘红亮,杨滨..HPLC法与UPLC法同时测定黄芩中5种黄酮类成分含量的比较[J].中国药房,2014,25(35):3293-3296,4.基金项目
国家自然科学基金资助项目(No.81202903) （No.81202903）
