中国药房2014，Vol.25Issue(35)：3302-3304,3.DOI:10.6039/j.issn.1001-0408.2014.35.13
不同产地斑蝥超微粉体的HPLC指纹图谱及斑蝥素的含量测定
Study on the HPLC Fingerprints of Spanish fly Ultra-fine Powder from Different Producing Areas and Content Determination of Cantharidin
摘要
Abstract
OBJECTIVE:To compare the difference of chemical components in Spanish fly from different producing areas on the basis of content determination and fingerprints of cantharidin,and to provide reference for quality control of S.fly from different producing areas and its ultra-fine powder.METHODS:Established the fingerprint method of S.fly ultra-fine powder.S.fly ultra-fine powder were determined by HPLC.Chromatographic Fingerprint Similarity Evaluation software was used to analyze the similarity of HPLC fingerprint.Cluster analysis was conducted in 10 batches of sample by using SPSS 13.0 software,and the content of cantharidin was determined.RESULTS:The fingerprints of S.fly ultra-fine powder from different producing areas were similar to each other.The content of cantharidin had little difference.Cluster analysis showed that there was little difference in S.fly from different producing areas.CONCLUSIONS:Established HPLC fingerprint is precise,reproducible and stable,and combined with content determination of cantharidin to provide reference for quality evaluation of S.fly and its ultra-fine powder.关键词
斑蝥/超微粉体/高效液相色谱法/指纹图谱/斑蝥素Key words
Spanish fly/ Ultra-fine powder/ HPLC/ Fingerprint/ Cantharidin分类
医药卫生引用本文复制引用
何杰,张水寒,李跃辉,万丹,黄江波..不同产地斑蝥超微粉体的HPLC指纹图谱及斑蝥素的含量测定[J].中国药房,2014,25(35):3302-3304,3.基金项目
湖南省自然科学基金资助项目(No.C190710) （No.C190710）
