中国药房2014，Vol.25Issue(35)：3305-3307,3.DOI:10.6039/j.issn.1001-0408.2014.35.14
不同产地白术醇提物的HPLC指纹图谱及聚类分析
HPLC Fingerprints and Cluster Analysis of Ethanol Extract from Atractylodes macrocephalae from Different Producing Areas
摘要
Abstract
OBJECTIVE:To evaluate the quality ofAtractylodes macrocephalae from the type of chemical component and peak area of characteristic component.METHODS:The HPLC fingerprint of ethanol extract from A.macrocephalae was established by HPLC.The determination was performed on CAPCELL PAK C18 (250 mm×4.6 mm,5 μm)column with mobile phase consisted of water-methanol (gradient elution) at the flow rate of 1.0 ml/min.The column temperature was 35℃,and detection wavelength was set at 220 nm.The sample size was 20 μl.TCM fingerprint similarity evaluation system,peak overlap rate calculation and hierarchical cluster analysis were used to assess it completely.RESULTS:10 common fingerprint peaks were detected in ethanol extract from 10 batches of A.macrocephalae,and atractylenolide Ⅲ,atractylenolide Ⅰ and atractylone were identified.The fingerprint peak overlap rate of A.macrocephalae ethanol extract from different producing areas was over 96％,and the similarity was over 90％.CONCLUSIONS:The established method can be use to evaluate the similarity ofA.macrocephalae from different producing areas in terms of chemical components and characteristic peak intensity,and provides foundation for the quality evaluation of A.macrocephalae and other medicinal materials.关键词
白术/指纹图谱/相似度评价/聚类分析Key words
Atractylodes macrocephalae/ Fingerprint/ Similarity evaluation/ Cluster analysis分类
医药卫生引用本文复制引用
李洪玉,董宇,陈莉君,寿旦,费莹..不同产地白术醇提物的HPLC指纹图谱及聚类分析[J].中国药房,2014,25(35):3305-3307,3.基金项目
浙江省自然科学基金资助项目(No.LY12B05004) （No.LY12B05004）
浙江省科技计划(No.2011F10017) （No.2011F10017）
浙江省中医药科技计划(No.2010ZB020) （No.2010ZB020）
