江苏大学学报（医学版）2014，Vol.24Issue(5)：394-398,5.DOI:10.13312/j.issn.1671-7783.y140213
薯蓣皂苷元体内外抑制胃癌增殖的机制
Mechanism underlying inhibition of migration of gastric carcinoma by diosgenin in vitro and vivo
霍中华 1胡君 1储著陵 1吕胜 1尹鹏 1章丽1
作者信息
- 1. 解放军第454医院普外科,江苏南京210002
- 折叠
摘要
Abstract
Objective:To investigate the principal pathway and preliminary mechanism by which diosgenin inhibits gastric carcinoma in vivo by treatment of human umbilical vein endothelial cells(HUVEC) and gastric carcinoma (BGC-823) cells for comparison.Methods:HUVEC and BGC-823 cells were treated with diosgenin at different concentrations and cell growth curves were plotted to observe the effects of diosgenin on proliferative activities of endothelial cells and gastric carcinoma cells; the scraping line method and canalization in vitro were used to detect the effects of diosgenin treatment on canalization of endothelial cells and migration ability of gastric carcinoma cells by treating HUVEC and BGC-823 cells with diosgenin at a certain concentration;a subcutaneous tumor model was established by inoculating nude mice with BGC-823 cells and diosgenin was administrated continuously to analyze its inhibitory effects on subcutaneous tumors.Results:Diosgenin had a significant effect on proliferation of endothelial cells in a concentration-dependent way,with a significant difference between the treatment groups and the control group at 48 or 72 h (P ＜ 0.05) ; and the same treatment had no evident effect on the proliferative activity of BGC-823 cells and effectively inhibited the migration of BGC-823 cells and significantly reduced the canalization of HUVEC cells in vitro; diosgenin exhibited a good inhibitory effect on subcutaneous tumors in the nude mice model of BGC-823 cells at an inhibitory rate of 38.2％ (P ＜ 0.05) in comparison with the model group.Conclusion:Diosgenin can eventually inhibit the proliferation of the subcutaneous tumor model by suppressing endothelial cell activity and consequently suppressing blood supply.关键词
薯蓣皂苷元/人血管内皮细胞/BGC-823细胞/增殖活性/体外成管/迁移/裸鼠成瘤Key words
diosgenin / human umbilical vein endothelial cells / BGC-823 cell / viability / capillary like tube formation/ cellular migration/nude mouse transplantation tumor分类
医药卫生引用本文复制引用
霍中华,胡君,储著陵,吕胜,尹鹏,章丽..薯蓣皂苷元体内外抑制胃癌增殖的机制[J].江苏大学学报（医学版）,2014,24(5):394-398,5.
