江苏大学学报（医学版）2014，Vol.24Issue(5)：399-402,407,5.DOI:10.13312/j.issn.1671-7783.y140060
弓形虫速殖子感染对HeLa细胞凋亡的影响
Effect of Toxoplasma gondii tachyzoite infection on HeLa cell apoptosis
摘要
Abstract
Objective:To explore HeLa cell apoptosis when infected with different number of T.gondii tachyzoites.Methods:HeLa cells were cultured in 24-wells plates for 16 h and incubated with different number of T.gondii tachyzoites.Mter infected for 4 h,the concentration of 0.5 μg/mL actinomycin D was added into the medium.The cells were collected in 8,12,24 and 36 h.The apoptosis of HeLa cell was determined by Hoechst 33258 stain and Annexin V-FITC/PI method.Results:When the rate of parasites/ cells (multiplicity of infection,MOI) was no more than 1 (MOI ≤ 1),the parasites could suppress the cell apoptosis.When MOI ＞ 1 and the infection time over 24 h,the parasites could accelerate the cells apoptosis.Conclusion:T.gondii tachyzoite infection could affect the HeLa cell appotosis in vitro,and the incidence was related to the number of tachyzoites.关键词
刚地弓形虫/细胞凋亡/HeLa细胞/放线菌素DKey words
Toxoplasma gondii / cell apoptosis / HeLa cells / actinomycin D分类
医药卫生引用本文复制引用
沈进,陈颖婷,吴亮,吴腊梅,王晓,陈盛霞,曹建平..弓形虫速殖子感染对HeLa细胞凋亡的影响[J].江苏大学学报（医学版）,2014,24(5):399-402,407,5.基金项目
国家自然科学基金资助项目(81301453) （81301453）
卫生部寄生虫病原与媒介生物学重点实验室开放课题(WSBKTKT201302) （WSBKTKT201302）
江苏大学高级人才启动基金资助项目(13JDG023,13JDG127) （13JDG023,13JDG127）
江苏省研究生创新计划资助项目(CX10B_282Z) （CX10B_282Z）
