中国妇幼健康研究Issue(5)：761-763,772,4.DOI:10.3969/j.issn.1673-5293.2014.05.017
靶向抑制存活素表达对卵巢癌细胞株生物学行为的影响和意义
Influence and significance of targeted inhibition of Survivin expression on biological behaviors of ovarian cancer cell line
摘要
Abstract
Objective To study the influence and significance of targeted inhibition of Survivin on proliferation, apoptosis, migration and invasion of SKOV3 cells by transfecting ovarian cancer cell line SKOV3 with antisense oligonucleotides aimed to Survivin ( Survivin ASODN) .Methods Survivin ASODN was introduced by LipofectamineTM2000 to transfect reagent into SKOV3 cells, and growth activities of SKOV3 cells were detected by tetrazolium bromide ( MTT) colorimetry.The changes of Survivn protein, cell cycle and apoptosis index ( AI) were detected by flow cytometry ( FCM) .The changes of invasion and migration were detected by Transwell.Results The protein level of Survivin was significantly down-regulated after transfection (t=7.82, P<0.01).Cell growth was significantly inhibited by various concentrations of ASODN (F=3.75,P<0.01).The cell survival rate was 49.50 ±20.76%after 48 hours of transfection with 600 nmol/L ASODN, which was close to IC50.AI increased significantly (t=6.37, P<0.05) but PI decreased significantly (t=-10.35,P<0.01), and more cells stayed G1/G0 phase (t=10.38, P<0.01).The migration ability and invasion ability of SKOV3 cells decreased (t value was 19.26 and 27.42, respectively, both P<0.01).Conclusion Survivin ASODN could inhibit proliferation, promote apoptosis, decrease the proportion of mitotic cells, reduce the migration ability and invasion ability and thus cure ovarian cancer.The targeted inhibition of Survivin will be an important therapy for ovarian cancer.关键词
卵巢癌/存活素/反义寡核苷酸/生物学行为Key words
varian cancer/Survivin/antisurvivin oligonucleotides ( ASODN)/biological behaviors分类
医药卫生引用本文复制引用
孙艳,王璟,赵向寨,底建敏..靶向抑制存活素表达对卵巢癌细胞株生物学行为的影响和意义[J].中国妇幼健康研究,2014,(5):761-763,772,4.基金项目
河北省自然基金资助项目 （）
