湖北医药学院学报2014，Vol.33Issue(5)：430-432,3.DOI:10.13819/j.issn.1006-9674.2014.05.004
甲硝唑及双氢青蒿素对阴道毛滴虫抗虫效果观察
The Effects of Metronidazole and Dihydroartemisinin on Trichomonas Vaginalis in vitro
摘要
Abstract
Objective To analyze the sensitivity of trichomonas vaginalis strains isolated in Shiyan district to metronidazole,to study the effect of Dihydroartemisinin (DHA) on low sensitive strains of metronidazole in vitro.Methods From 2009 to 2011,total 25 strains of trichomonas vaginalis were isolated from gynecological clinic service in Taihe hospital,and then cultured and purified in vitro.All the strains was administrated by metronidazole with concentrations of 292.1,182.6,146.1,91.3,45.6,22.8,1 1.4,5.7 μmol/L,and then the death rate of strains were determined at 4,8,12,24 h,respectively.The minimum lethal concentration(MLC) in all 25 strains were analyzed in the given time.If the MLC of the strain in 24 h was much more than 22.8 μmol/L,the death rate of the strain should be determined after treatment with DHA under the concentrations of 5.3,3.5,1.8 mmol/L at 4,8,12,24 h,respectively.Results In addition to one strain,the other 24 strains were sensitive to metronidazole and MLC in 24 h were 11.4 to 22.8 μmol/L,however the MLC of the only non-sensitive strain was 22.8 to 45.6 μmol/L in 24 h.The death rate of the non-sensitive strain had no significant difference after treatment with 22.8 or 45.6 μmol/L of metronidazole for 24 h(P ＞ 0.05).When administrating with 3.5 and 5.3 mmol/L of DHA for 4,8,12 h,the death rates in the non-sensitive strain were all significant difference in the given time(P ＜0.05),and 24 h MLC was 3.5 to 5.3 mmol/L.Conclusion Trichomonas vaginalis strains isolated from Shiyan district were mostly sensitive to metronidazole,but individual strain was low sensitive to metronidazole.DHA has obvious effect on low sensitive strain and its MLC was 3.5 to 5.3 mmol/L.关键词
阴道毛滴虫/甲硝唑/双氢青蒿素/体外实验Key words
Trichomonas vaginalis/ Metronidazole/ Dihydroartemisinin/ In vitro引用本文复制引用
杨树国,郭鄂平,王娅..甲硝唑及双氢青蒿素对阴道毛滴虫抗虫效果观察[J].湖北医药学院学报,2014,33(5):430-432,3.基金项目
湖北医药学院研究生启动金项目(2008QDJ15) （2008QDJ15）
湖北医药学院校级重点学科建设项目(2014SKJSXJ04) （2014SKJSXJ04）
