中药材2014，Vol.37Issue(9)：1562-1565,4.DOI:10.13863/j.issn1001-4454.2014.09.014
生、熟大黄泻下作用及其机制研究
Purgative Activity Difference and Mechanism of Raw and Processed Rhei Radix et Rhizoma
摘要
Abstract
Objective:To investigate the purgative activity difference and mechanism of raw and processed Rhei Radix et Rhizoma.Methods:Mail mice were divided into 9 groups:normal group (saline),model group,Tongbianling group (1 g/kg),raw Rhei Radix et Rhizoma groups(5.00,2.50 and 1.25 g/kg),processed Rhei Radix et Rhizoma groups(5.00,2.50 and 1.25 g/kg).After oral administration for 5 days,the diarrhea experiment and intestinal propelling test were carried out to evaluate the purgative effect difference of raw and processed Rhei Radix et Rhizoma.Wistar rats were divided into normal group(normal saline),model group,Tongbianling group(0.5 g/kg),raw Rhei Radix et Rhizoma group(1.25 g/kg),processed Rhei Radix et Rhizoma group(1.25 g/kg).The changes of levels of gastin (GAS),motilin (MTL),vasoactive peptide (VIP),neurotensin (NT),somatostatin (SS),acetylcholinesterase (AchE),substance P(SP) and endothelin (ET-1)in blood of the rats and gastin(GAS),motilin(MTL),vasoactive peptide(VIP) and neurotensin(NT) in colon of the rats were detected.Result:The levels of GAS,MTL,VIP,NT,SS,AchE,SP and ET-1 in serum of raw Rhei Radix et Rhizoma group,and GAS,MTL and VIP in colon of raw Rhei Radix et Rhizoma group were different obviously comparing with the processed one(P ＜ 0.01).Conclusion:The raw Rhei Radix et Rhizoma and the processed one had obvious differences in regulating intestinal gastrointestinal hormone and neurotransmitter.This effect may be the reason or one of the reasons for purgative activity difference between raw and processed Rhei Radix et Rhizoma.关键词
生大黄/熟大黄/泻下作用/胃肠激素/肠神经递质Key words
Raw Rheum officinale Baill.rhizome / Processed rhubarb / Purgative activity / Gastrointestinal hormone / Gastrointestinal neurotransmitter分类
医药卫生引用本文复制引用
吴晓青,胡昌江,赵玲,耿媛媛,潘新,胡麟..生、熟大黄泻下作用及其机制研究[J].中药材,2014,37(9):1562-1565,4.基金项目
中医药行业科研专项(20110700710) （20110700710）
