中药材2014，Vol.37Issue(9)：1570-1573,4.DOI:10.13863/j.issn1001-4454.2014.09.016
苗药矮地茶的HPLC指纹图谱研究
Study on HPLC Fingerprint of Miao Medicine Ardisia japonica
摘要
Abstract
Objective:To establish the HPLC fingerprint of Ardisia japonica.Methods:HPLC analysis was performed on a Diamonsil C18 column(250 mm × 4.6 mm,5 μm)with the eluting system of gradient consisted of methanol-0.1％ H3PO4.The flow rate was 1.0 mL/min.The column temperature was maintained at 35 ℃ and the detection wavelength was 250 nm.Results:The HPLC fingerprint was established and 10 batches of samples with 32 common peaks were compared.Four peaks were identified as gallic acid,bergenin,chlorogenic acid and quercitrin.Conclusion:The method with good reproducibility is simple and accurate,which can be used for determination of HPLC fingerprint and quality control of Ardisia japonica.It provides scientific basis of future study of Ardisia japonica.关键词
苗药/矮地茶/HPLC指纹图谱Key words
Miao medicine/Ardisia japonica (Thunb.) Blume / HPLC fingerprint分类
医药卫生引用本文复制引用
徐昌艳,张丽艳,汪毅,魏升华,覃容贵,迟明艳..苗药矮地茶的HPLC指纹图谱研究[J].中药材,2014,37(9):1570-1573,4.基金项目
贵阳市科技局现代药业计划项目(筑科合同[2011204]13号) （筑科合同[2011204]13号）
贵阳市科技局现代药业计化项目(筑科合同[2013204]7-2号) （筑科合同[2013204]7-2号）
