中药材2014，Vol.37Issue(9)：1604-1608,5.DOI:10.13863/j.issn1001-4454.2014.09.025
指纹图谱与一测多评法相结合测定五味子种子中木脂素类成分
Determination of Lignanoids in Seeds of Schisandra chinensis by Combinative Methods of Fingerprint and QAMS
摘要
Abstract
Objective:To establish a fingerprint of seeds of Schisandra chinensis (SSC) and develop a method of quantitative analysis of multi-components by single marker(QAMS) for simultaneous determining six lignanoids in SSC.Methods:Eleven batches of SSC were determined by HPLC and a common mode of fingerprint has been established.A method was developed for QAMS to determine schizandrol A,schizandrol B,schisantherin A,deoxyschizandrin,schizandrin B and schizandrin C in SSC.Schizandrol A was selected as internal reference;the relative correction factors (RCF)of other five lignanoids to the internal reference were calculated.The contents of the six lignanoids in eleven batches of SSC were determined by both external standard method and QAMS.The QAMS method was evaluated by comparison of its assay results with that of external standard method.Results:There were 24 common peaks in fingerprints of eleven batches of SSC,six of them were identified.The similarities of fingerprints of eleven batches of SSC were over 0.980.The established RCF had a good reproducibility.No significant differences were found between the quantitative results of external standard method and QAMS.Conclusion:The developed method is accurate,feasible,and can be used for the qualitative and quantitative analysis of lignanoids in SSC.关键词
五味子种子/指纹图谱/一测多评法/木脂素Key words
Seeds of Schisandra chinensis (Turcz.) Baill./ Fingerprint / Quantitative analysis of multi-components by single marker(QAMS) / Lignanoid分类
医药卫生引用本文复制引用
窦志华,安莉萍,罗琳,居宇峰,乔进,陈敏,缪应祥..指纹图谱与一测多评法相结合测定五味子种子中木脂素类成分[J].中药材,2014,37(9):1604-1608,5.基金项目
南通市应用研究计划项目(K2008027) （K2008027）
江苏省药学会科研项目(JS2012-04) （JS2012-04）
