中药材2014，Vol.37Issue(9)：1636-1640,5.DOI:10.13863/j.issn1001-4454.2014.09.033
HPLC法同时测定大鼠口服墨旱莲提取物后血浆中四种黄酮类化合物
HPLC Determination of Four Flavonoids in Rat Plasma After Oral Ecliptae Herba Extract
摘要
Abstract
Objective:To establish an HPLC method for determination of four flavonoids in rat plasma after oral Ecliptae Herba extract.Methods:HPLC separation was carried out in a Neocleodur 100-5 C18 column(250 mm × 4.6 mm,5 μm)with mobile phase A(consisting of methanol-0.2％ phosphoric acid =50∶ 50)and mobile phase B (acetonitrile)with gradient elution at the flow rate of 1.0 mL/min.The detection wavelength was 350 nm and the column temperature was maintained at 40 ℃.Results:There were good linear relationships between concentration and peak area ratio of luteolin,apigenin,scutellarein and diosmetin in the ranges of 0.02 ～50 μg/ mL with the recovery rates of 71.5％ ～90.2％.Conclusion:This method is rapid,accurate and reproducible for determination of lnteolin,apigenin,scutellarein and diosmetin in rat plasma and study on pharmacokinetics of Ecliptae Herba extract.关键词
旱莲草提取物/木犀草素/芹菜素/野黄芩素/香叶木素/高效液相色谱法/血药浓度Key words
Ecliptae Herba extract / Luteolin / Apigenin / Scutellarein / Diosmetin / HPLC / Plasma concentration分类
医药卫生引用本文复制引用
邓斌,韦炳华,黄盛超,张志豪,唐蕾..HPLC法同时测定大鼠口服墨旱莲提取物后血浆中四种黄酮类化合物[J].中药材,2014,37(9):1636-1640,5.基金项目
广州市重大科技专项计划项目(2008A1-E4101-7) （2008A1-E4101-7）
广东省中医药局课题(2009159) （2009159）
