国际医药卫生导报2015，Vol.21Issue(1)：9-11,15,4.DOI:10.3760/cma.j.issn.1007-1245.2015.01.003
DNA聚合酶β在宫颈上皮内瘤变和宫颈癌中的表达及临床意义
Expression of pol(B) in tissues of cervical intraepithelial neoplasia and cervical carcinoma and its clinical significance
摘要
Abstract
Objective To investigate the expression of pol(B) of in tissues of cervical intraepithelial neoplasia (CIN) and cervical carcinoma (CC) and its clinical significance.Methods The expression of pol(B) mRNA in normal cervical,CIN,and CC tissues were examined by RT-PCR assay,and that of pol(B)protein by Western Blot assay.Results The expressions of pol(B) mRNA of in normal cervix,CIN Ⅰ,CIN Ⅱ,CIN Ⅲ,and CC tissues were (0.23 ±0.08),(0.25 ±0.11),(0.36 ±0.18),(0.62 ±0.22),and (0.85±0.28),respectively; there were statistical differences in the expression of pol(B) mRNA between normal cervix,CIN Ⅱ,CIN Ⅲ,and CC (P＜0.05).The expressions of pol(B) protein in normal cervical,CIN Ⅰ,CIN Ⅱ,CIN Ⅲ,and CC tissues were (19±8)％,(18±6)％,(43±17)％,(72±26)％,and (93±31)％,respectively; there was statistical differences in the expression of pol(B) protein between normal cervical,CIN Ⅱ,CIN Ⅲ,and CC (P＜0.05).The expression of pol(B) mRNA and protein of in the tissues of CIN Ⅰ and normal cervix was not significantly different(P＜0.05).Conclusions The expression of pol(B) mRNA and protein increases with the progression of cervical lesions.Pol(B) might play an important role in the genesis and development of CC,and it could be the indicator in the development and prognosis of CC.关键词
DNA聚合酶β/宫颈上皮内瘤变/宫颈癌Key words
pol(B)/ CIN/ CC引用本文复制引用
段娥,李英勇..DNA聚合酶β在宫颈上皮内瘤变和宫颈癌中的表达及临床意义[J].国际医药卫生导报,2015,21(1):9-11,15,4.基金项目
江门市卫生局立项课题(12A057) （12A057）
