中国临床药理学与治疗学2014，Vol.19Issue(12)：1342-1346,5.
乙醛脱氢酶2在糖尿病大鼠致心肌损伤中的变化
Changes of aldehyde dehydrogenase 2 on myocardial injury in diabetic rats
摘要
Abstract
AIM:To investigate the changes of aldehyde dehydrogenase 2 (ALDH2) on myocardial injury in different stages of diabetic rats and analyze the related mechanism.METHODS:Diabetic (DM) model in SD rat was induced by a single intraperitoneal injection of 55 mg/kg streptozoticin (STZ).DM rats were divided into fourth week (DM4W),eighth week (DM8W) and twelfth week (DM12W) groups.The ventricular hemodynamic parameters were recorded,plasma interleukin-1 (IL-1),interleukin-4 (IL-4) and cardiac 4-hydroxynon-2-enal (4-HNE) levels were determined by enzyme-linked immuno sorbent assay (ELISA),cardiac ALDH2 activity was measured.The expressions of Bax and Bcl-2 at mRNA level of left anterior myocardium were detected by RT-PCR.RESULTS:In contrast to control group,in DM4W,DM8W and DM12W groups,left ventricular development pressure (LVDP),heart rate (HR) and left ventricular work (RPP) were decreased (P ＜ 0.01),plasma IL-1 level was increased while IL-4 level was decreased (P＜ 0.01).Cardiac 4-HNE level was increased,and ALDH2 activity was decreased(P＜0.01).The expression of Bax mRNA level was increased (P ＜ 0.01),Bcl-2 mRNA level was decreased(P＜0.01) and Bcl-2/Bax mRNA were decreased(P＜0.01).And with the development of diabetic progression,LVDP,HR,RPP,IL-4,ALDH2 activity and Bcl-2/Bax mRNA were further reduced (P ＜ 0.05 or P＜0.01).4-HNE and IL-1 levels were increased(P ＜ 0.05 or P ＜ 0.01).CONCLUSION:With the development of diabetes,cardiac ALDH2 activity was further decreased,which may be related to the increase of inflammatory injury and the happening of apoptosis.关键词
糖尿病/乙醛脱氢酶2/4-羟壬烯醛/炎性损伤/细胞凋亡Key words
diabetes/ aldehyde dehydrogenase 2 / 4-hydroxynon-2-enal / inflammatory injury/ apoptosis分类
医药卫生引用本文复制引用
史晓俊,康品方,高琴,叶红伟,李正红,汤阳,王洪巨..乙醛脱氢酶2在糖尿病大鼠致心肌损伤中的变化[J].中国临床药理学与治疗学,2014,19(12):1342-1346,5.基金项目
国家自然科学基金资助项目(81000074) （81000074）
蚌埠医学院科技发展基金(Bykf13A16) （Bykf13A16）
