江苏大学学报（医学版）2015，Vol.25Issue(1)：22-26,32,6.DOI:10.13312/j.issn.1671-7783.y140254
下调丙酮酸激酶2表达对膀胱癌T24细胞株生物学特性的影响
Expression of PKM2 in bladder carcinoma and its effect of down-regulation on biological characteristics of bladder carcinoma cell line T24
郭科 1莫乃新1
作者信息
- 1. 江苏大学附属武进医院泌尿外科,江苏常州213002
- 折叠
摘要
Abstract
Objective:To investigate the effects and possible mechanism of PKM2 on the proliferation,invasion,migration of human bladder cancer cell lines T24 by using RNA interfering methods.Methods:The expression of PKM2 protein in 50 cases of bladder tumor tissues and 15 cases of normal bladder tissues was detected by immunohistochemistry.PKM2 siRNA and control siRNA were transfected to T24 cells by Li-pofectamine 2000 respectively.The stable PKM2 siRNA and RNAi-Vector cell lines were obtained after screening and identification.Untransfected and transfected nonsense siRNA cells were used as blank control group and negative control group,the cells transfected PKM2 siRNA were as the experimental group.Real time-RCR and Western blot were used to measure the PKM2 expression at mRNA and protein levels.Cell proliferation rate were determined by MTT assay.Cell migration and invasion were determined by cell scratch experiment and Transwell invasive assays.Results:The positive expression of PKM2 protein in bladder cancer tissues was significantly higher than that of normal bladder tissues,and correlated with the pathological grade and clinical stage.In the PKM2 siRNA stable cell lines T24,the expression of PKM2 mRNA and protein were significantly decreased compared with these in blank control group and negative control group(P ＜ 0.05).The cell proliferation,the number of transmembrane cells and the ability of migration of PKM2 siRNA bladder cancer cell line were markedly reduced (P ＜ 0.05).Conclusion:The PKM2 overexpressed may play an essential role in the occurrence and development of bladder cancer,down regulation of PKM2 expression can inhibit the proliferation,migration and invasion of bladder cancer cell.关键词
丙酮酸激酶2/膀胱癌/细胞增殖/细胞侵袭/细胞迁移Key words
pyruvate kinase M2/bladder cancer/cell proliferation/cell invasion/cell migration分类
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郭科,莫乃新..下调丙酮酸激酶2表达对膀胱癌T24细胞株生物学特性的影响[J].江苏大学学报（医学版）,2015,25(1):22-26,32,6.
