江苏大学学报（医学版）2015，Vol.25Issue(2)：109-113,5.DOI:10.13312/j.issn.1671-7783.y140335
雷马曲班对嘌呤霉素氨基核苷诱导的足细胞凋亡的影响
Effect of ramatroban on podocyte apoptosis induced by puromycin aminonucleoside
摘要
Abstract
Objective:To observe the uhrastructure changes of ramatroban on podocyte apoptosis induced by puromycin aminonucleoside (PAN).Methods:The conditionally immortalized murine podocytes were randomly divided into four groups:control group,the podocytes were incubated with RPMI-1640;model group,the podocytes exposed to 100 mg/mL PAN;treatment group,the podocytes were pretreated with 100 μg/mL ramatroban for 1 h before 100 mg/mL PAN exposure;ramatroban group,podocytes exposed to 100 μg/mL ramatroban.Each group cells were collected at 24 h and 48 h,respectively.The level of podocytes apoptosis was detected by FITC-Annexin V/PI assay under fluorescence microscope.The ultrastructure changes of podocyte morphology were observed with transmission electron microscope.Results:Compared with control group,the apoptosis was significantly increased in model group and treatment group (P ＜ 0.05) at 24 and 48 h.No obvious apoptosis was observed in the ramatroban group(P ＞ 0.05),while the apoptosis of treatment group was obviously decreased at 24 h compared with model group (P ＜ 0.05),the apoptosis in treatment group at 48 h was significantly increased than control group at 48 h.Compared with model group,the cell ultrastructural damage in treatment group was significantly reduced after treatment with ramatroban at 24 and 48 h;TEM displayed cytoplasmic vacuolar degeneration and apoptotic bodies obviously decreased.Conclusion:Ramatroban could reduce PAN-induced podocyte apoptosis rate and cell injury.关键词
足细胞/凋亡/嘌呤霉素氨基核苷/雷马曲班Key words
podocyte/apoptosis/puromycin aminonucleoside/ramatroban分类
医药卫生引用本文复制引用
朱小东,孔程,孔维信,陆志峰,邹锋..雷马曲班对嘌呤霉素氨基核苷诱导的足细胞凋亡的影响[J].江苏大学学报（医学版）,2015,25(2):109-113,5.基金项目
苏州市科技计划资助项目(SYSD2011070) （SYSD2011070）
苏州工业园区孵化项目 （）
