江苏大学学报（医学版）2015，Vol.25Issue(2)：123-127,5.DOI:10.13312/j.issn.1671-7783.y150003
人PD-L1基因转染细胞株的构建及其对活化的Jurkat细胞增殖和凋亡的影响
Construction of human PD-L1 gene transfected cell line and its biological function on the proliferation and apoptosis of activated Jurkat cell
摘要
Abstract
Objective:To construct a gene transfected cell line that stably expressing the human PD-L1 and observe its biological function on the proliferation and apoptosis of activated Jurkat cell.Methods:Human PD-L1 gene was sub-cloned into retroviral expressing vector pEGZ-Term-R.The recombinant plasmid together with its helper virus vector was cotransfected into the 293T cells.The L929 cells were infected with the supernatant of the transfected 293T cells,and then were selected with G418.The G418 resistant cells were harvested for screening PD-L1 expression by flow cytometry.L929/PD-L1 cell was cocultivated with PHA stimulated Jurkat cell.The biological effect on proliferation and apoptosis of Jurkat cell was analyzed by cell counting and flow cytometry.Results:Gene transfected line L929/PD-L1 that stably expressed the human PD-L1 was established.Membrane PD-1 expression on Jurkat cell was induced by PHA administration.L929/PD-L1 inhibited the proliferation and induced the apoptosis of activated Jurkat cell.Conclusion:Gene transfected cell line that stably expressed the human PD-L1 was established successfully.The PD-L1 on the cell line inhibited the proliferation and induced the apoptosis of activated Jurkat cell.关键词
PD-L1/基因转染细胞株/Jurkat细胞Key words
PD-L1/gene transfected cell line/Jurkat cell分类
医药卫生引用本文复制引用
宋莉,葛彦,曹莎莎,徐永芳,潘建忠,谢炜,居颂文,居颂光..人PD-L1基因转染细胞株的构建及其对活化的Jurkat细胞增殖和凋亡的影响[J].江苏大学学报（医学版）,2015,25(2):123-127,5.基金项目
国家自然科学基金资助项目(31370887,81373149,31200661) （31370887,81373149,31200661）
江苏省自然科学基金资助项目(BK2012621) （BK2012621）
苏州市科技发展计划项目(SYS201363) （SYS201363）
