江苏大学学报（医学版）2015，Vol.25Issue(2)：128-132,5.DOI:10.13312/j.issn.1671-7783.y140295
Toll样受体4表达与膝骨关节炎软骨细胞凋亡的关系
Toll-like receptor 4 and knee osteoarthritis chondrocytes apoptosis
钱臣 1卞晓星2
作者信息
- 1. 江苏大学附属武进医院骨科,江苏常州213002
- 2. 江苏大学附属武进医院脑外科,江苏常州213002
- 折叠
摘要
Abstract
Objective:Discuss the relationship between human knee osteoarthritis chondrocytes apoptosis and Toll-like receptor 4 (TLR4) expression,to see whether TLR4 signaling pathways effect or not on pathogenesis of osteoarthritis.Methods:Jonit cartilage was shredded after harvested from the patients of osteoarthritis undergoing the replacements of knee,and chondrocytes were isolated and cultured by the way of enzymatic digestion.The third-passage cells in the logarithmic growth phase were cultured in vitro and randomly divided into Z-VAD-FMK (ZVF) group,camptothecin group,control group.TUNEL method was used to assay apoptosis index of the chondrocytes.Immunohistochemical staining and Western blotting were adopted to observe TLR4 expression.The chondrocytes were randomly divided into control group and TLR4 inhibitor group.Flow cytometry instrument and Caspase-3 kit were used to assay apoptosis index of the chondrocytes.Results:The results of TLR4 immunohistochemical staining and Western blotting were that the expression of TLR4 was induced by camptothecin but reduced by ZVF,in parallel with the results of cells apoptosis index.In addition,chondrocyte apoptosis was reduced by incubation with TLR4-inhibitor.The results of Flow cytometry instrument and Caspase-3 kit were consistent.Conclusion:Apoptosis chondrocytes could up-regulate TLR4 expression.There was a positive correlation between TLR4 and apoptosis.TLR4-inhibitor could reduce chondrocytes apoptosis in vitro model.TLR4 signaling pathways involved in chondrocytes apoptosis and played an important role on osteoarthritis.关键词
膝骨关节炎/Toll样受体4/软骨细胞/细胞凋亡Key words
knee osteoarthritis/Toll-like receptor 4/chondrocyte/apoptosis分类
医药卫生引用本文复制引用
钱臣,卞晓星..Toll样受体4表达与膝骨关节炎软骨细胞凋亡的关系[J].江苏大学学报（医学版）,2015,25(2):128-132,5.
