江苏大学学报（医学版）2015，Vol.25Issue(2)：133-136,141,5.DOI:10.13312/j.issn.1671-7783.y140195
积雪草酸对结肠癌HCT116细胞增殖及自噬水平的影响
Inhibition effect of asiatic acid on the proliferation and autophagy of HCT116 colon cancer cell
耿骥 1郭文洁 1刘佳 1谈德斐 1Daniel Boison 1高静1
作者信息
摘要
Abstract
Objective:To investigate the effect of asiatic acid on the proliferation and autophagy level in HCT116 cells.Methods:After treating HCT116 cells with 10,30,60,90 μmol/L asiatic acid for 24 h and 48 h,the cell viability ratio was detected by MTTF method.The formation of autophagy vesicles were observed by MDC autophagy specific fluorescence staining.The expressions of LC3-Ⅱ,p62,p-mTOR,p-4EBP1 were detected by Western blotting.Results:Asiatic acid inhibited the proliferation of HCT116 when they were treated with 90 μmol/L.Autophagy was induced by 30,60,90 μmol/L of asiatic acid and the autophagic marker LC3-Ⅱ was strongly expressed at 90μmol/L asiatic acid treatment for 4,8,12 h as shown by Western blotting.A combination of 5 μmol/L chloroquine with 90 μmol/L of asiatic acid,promote autophagic flux in a dose-dependent manner.Conclusion:Asiatic acid can inhibit the proliferation of HCT116 cells obviously and promote autophagic flux by inhibiting mTOR-4EBP1 signal pathway in a time-and dose-dependent manner.关键词
积雪草酸/结肠癌/细胞自噬/氯喹Key words
asiatic acid/colon cancer/autophagy/chloroquine分类
医药卫生引用本文复制引用
耿骥,郭文洁,刘佳,谈德斐,Daniel Boison,高静..积雪草酸对结肠癌HCT116细胞增殖及自噬水平的影响[J].江苏大学学报（医学版）,2015,25(2):133-136,141,5.
