中国美容医学Issue(6)：32-34,3.
大气细颗粒物对小鼠皮肤组织中表皮生长因子受体mRNA表达的影响
The influence of atmospheric fine particles on the expression of EGFR mRNA in mouse skin tissue
韩雪 1梁文艳 2梁文丽 1张苑 1孙乐栋1
作者信息
- 1. 南方医科大学珠江医院 广东 广州 510280
- 2. 北京林业大学资源与环境学院 北京 100083
- 折叠
摘要
Abstract
Objective To study the influence of atmospheric fine particles on the expression of EGFR mRNA in mouse skin tissue and to explore the influence of atmospheric fine particles of skin aging. Methods 40 female BALB/c mice were randomly divided into 4 groups including 1 saline control group and 3 PM2.5 exposure groups(1.6, 8.0 and 40.0mg/kg body weight, respectively).Each exposure group received intratracheal instillation once per day for 3 consecutive days.At the 24h after the last administration,skin tissueand were collected to detect the expression of EGFR mRNA by real-time fluorescent quantitative PCR. Results Compared with control group,the expression of EGFR mRNA increased significantly in the 8.0 and 40.0mg/kg PM2.5 exposure groups (P<0.05),and positively related to dosage of PM2.5. Conclusion The 8.0 and 40.0mg/kg PM2.5 exposure can induce the expression of EGFR mRNA and promote the occurrence of skin aging.关键词
大气细颗粒物/表皮细胞生长因子受体/实时荧光定量PCRKey words
atmospheric fine particles/epidermal growth factor receptor/Real-Time PCR分类
生物科学引用本文复制引用
韩雪,梁文艳,梁文丽,张苑,孙乐栋..大气细颗粒物对小鼠皮肤组织中表皮生长因子受体mRNA表达的影响[J].中国美容医学,2015,(6):32-34,3.
