国际医药卫生导报2015，Vol.21Issue(12)：1644-1647,4.DOI:10.3760/cma.j.issn.1007-1245.2015.12.004
支气管哮喘患者血清IL-33、IL-35水平及相关性分析
The expression of serum interleukin-33, interleukin-35 in patients with bronchial asthma and their correlation
摘要
Abstract
Objective To investigate the expression of serum interleukin-33 (IL-33),interleukin-35 (IL-35) in patients with bronchial asthma and their correlation.Methods The expression of serum IL-33,IL-35 of 81 patients with moderate or severe bronchial asthma in acute episode not caused by bacterial infection were detected with ELISA before treatment,along with 80 normal controls.The numbers of white blood cell (WBC),absolute neutrophil count (N),eosinophil absolute value (EOS) were detected by blood cell analyzer.In cell culture experiments,the plasma sample was divided into 3 groups:control group,cell stimulating hormone group,and IL-35 group.After cell culture experiments,the expression of plasma IL-33 in each group was measured by ELISA.Compared the expression of serum IL-35,IL-33,WBC,N,EOS in patients with bronchial asthma in acute episode and normal controls,analyzed the correlation between IL-35 and IL-33.Results ① The numbers of WBC,N,EOS of bronchial asthma group were higher than those of control group [(7.82±2.93)× 109/L vs.(6.38±2.14)× 109/L;(5.63± 1.47)× 109/L vs.(4.57± 1.22)× 109/L;(0.43±0.27)× 109/L vs.(0.22±0.15) × 109/L;P＜0.001].② The expression of serum IL-33 of bronchial asthma group was significantly higher than that of control group [(217.26±52.31)ng/L vs.(105.43±31.64)ng/L],the expression of serum IL-35 was significantly lower than that of control group [(156.71±24.29)ng/L vs.(311.62±35.28)ng/L],with statistically significant differences (P＜0.05).③The expression of serum IL-33 of patients with bronchial asthma undergoing IL-35 stimulating was lower that of patients with bronchial asthma undergoing cell stimulating hormone [(15.08±1.92)ng/L vs.(57.33±15.62)ng/L],with statistically significant difference (P＜0.05).Conclusion IL-35,IL-33 were involved in the pathogenesis of bronchial asthma.Cytokines regulating unbalance existed in acute episode of bronchial asthma.关键词
支气管哮喘/白介素33/白介素35Key words
Bronchial asthma/Interleukin-33/Interleukin-35引用本文复制引用
陈杰,彭健,张卓然,程国平..支气管哮喘患者血清IL-33、IL-35水平及相关性分析[J].国际医药卫生导报,2015,21(12):1644-1647,4.基金项目
湖北省自然科学基金指导性项目(2013CFC057) （2013CFC057）
黄石市医药卫生科研项目(2013-34) （2013-34）
