中医药导报2015，Vol.21Issue(14)：10-13,18,5.
脾虚状态对高脂血症大鼠血清低密度脂蛋白亚类分布的影响
Effects of Spleen Deficiency on Serum LDL Subfractions Distribution of Hyperlipidemia Rats
摘要
Abstract
Objective:To observe the characteristics of LDL subfractions distribution of apleen deficiency combined hyperlipidemia rats serum,then to analysis the influences of spleen deficiency on serum LDL subfractions distribution of hypedipidemia rats at the level of subfractions.Method:The 30 SPF SD rats were randomized divided into blank control group,hyperlipemia group and spleen deficiency combined hypedipidemia group.Hypedipidemia group were fed with high fat diet,while spleen deficiency combined hyperlipidemia group using overstrain and improper diet with high fat diet to feed.The levels of serum lipid were tested by biochemical methods.Using phloroglucinol method to test D-xylose excretion rate.HE and oil red staining method was used to observe the liver injury.Using Lipoprint classification system to test lipoprotein LDL subclasses.Results:Compared with the blank group,the serum TC and LDL-C levels increased significantly while HDL-C,AMY,D-xylose excretion rate were significantly reduced,the liver cells see a lot of lipid deposited in the hypedipemia group and spleen deficiency combined hyperlipidemia group.Compared with the hypedipemia group,the serum TC and LDL-C levels increased significantly while HDL-C,AMY,D-xylose excretion rate were significantly reduced in the spleen deficiency combined hyperlipidemia group.The results of Lipoprint lipoprotein classification system showed that:Compared with the control group,the serum large particles LDL and the small dense LDL levels were significantly increased in the hyperlipemia group and spleen deficiency combined hypedipidemia group.Compared with the hyperlipemia group,the serum small dense LDL levels were significantly increased.Conclusion:The distribution of serum LDL subfractions distribution anomaly in the hypedipemia group and spleen deficiency combined hypedipidemia group rats,and the subfractions of the latter changed more.关键词
低密度脂蛋白/亚类/高脂血症/脾虚/大鼠Key words
Low Density Lipoprotein/Subclasses/Hyperlipidemia/Spleen Deficiency/Rats分类
医药卫生引用本文复制引用
朱美林,贾连群,陈阳,杜莹,任路..脾虚状态对高脂血症大鼠血清低密度脂蛋白亚类分布的影响[J].中医药导报,2015,21(14):10-13,18,5.基金项目
国家自然科学基金青年基金项目(81202834) （81202834）
沈阳市科技局计划项目(F12-277-1-49) （F12-277-1-49）
