摘要
Abstract
Objective:To develop a RP-HPLC method for the determination of chlorogenic acid and linarin in Yejuhua Shuan.Method:Agilent XDB-C18 (4.6 mm×250 mm,5 μm) column was used,the mobile phase was CH3OH-H2O-HAc,gradient elution.The flow rate was 1.0 mL/min,detection wavelenghth was 334 mn,and the column pemprature was 30 ℃.Results:The linear relationship of chlorogenic acid and linarin were in the ranges of 4.32～103.7,12.64～303.4 μg/mL;the average recovery of chlorogenic acid and linarin 100.2％,99.5％,with RSD were 1.9％,1.5％,respectively.Conclusion:The method is accurate,rapid,and can be used for determination of chlorogenic acid and linarin in Yejuhua Shuan,and to improve the quality standards.关键词
RP-HPLC/野菊花栓/绿原酸/蒙花苷Key words
RP-HPLC/Yejuhua Shuan/Chlorogenic Acid/Linarin分类
医药卫生
